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. 2023 Sep;27(9):5041-5048.
doi: 10.1007/s00784-023-05123-x. Epub 2023 Jul 8.

Histological evaluation following treatment of recession-type defects with coronally advanced flap and a novel human recombinant amelogenin

Tali Chackartchi  1 , Dieter D Bosshardt  1   2 , Jean-Claude Imber  3 , Alexandra Stähli  3 , Hagit Sacks  4 , Katalin Nagy  5 , Anton Sculean  6
Affiliations

Histological evaluation following treatment of recession-type defects with coronally advanced flap and a novel human recombinant amelogenin

Tali Chackartchi et al. Clin Oral Investig. 2023 Sep.

Abstract

Objectives: To histologically evaluate the effects of a novel human recombinant amelogenin (rAmelX) on periodontal wound healing / regeneration in recession-type defects.

Materials and methods: A total of 17 gingival recession-type defects were surgically created in the maxilla of three minipigs. The defects were randomly treated with a coronally advanced flap (CAF) and either rAmelX (test), or a CAF and placebo (control). At three months following reconstructive surgery, the animals were euthanized, and the healing outcomes histologically evaluated.

Results: The test group yielded statistically significantly (p = 0.047) greater formation of cementum with inserting collagen fibers compared with the control group (i.e., 4.38 mm ± 0.36 mm vs. 3.48 mm ± 1.13 mm). Bone formation measured 2.15 mm ± 0.8 mm in the test group and 2.24 mm ± 1.23 mm in the control group, respectively, without a statistically significant difference (p = 0.94).

Conclusions: The present data have provided for the first-time evidence for the potential of rAmelX to promote regeneration of periodontal ligament and root cementum in recession-type defects, thus warranting further preclinical and clinical testing.

Clinical relevance: The present results set the basis for the potential clinical application of rAmelX in reconstructive periodontal surgery.

Keywords: Gingival recessions; Periodontal regeneration; Periodontal wound healing; Recombinant Amelogenin.

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Conflict of interest statement

Tali Chackartchi: has no conflict of interest related to this study.

Dieter D. Bosshardt: has no conflict of interest related to this study.

Jean-Claude Imber: has no conflict of interest related to this study.

Alexandra Stähli: has no conflict of interest related to this study.

Hagit Sacks: is employed by Prudentix Ltd, Israel.

Katalin Nagy: has no conflict of interest related to this study.

Anton Sculean: has no conflict of interest related to this study.

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Surgical pictures illustrating the procedure in the test group. (a) After Flap elevation and defect creation (b) application of the biomaterial (rAmelX) (c) after wound closure
Fig. 2
Fig. 2
Histometrical landmarks. aJE, apical end of junctional epithelium; aN, apical notch; CEJ, Cemento-enamel junction (CEJ); GM, Gingival margin; hNB, highest point of new bone; hNC, highest point of new cementum
Fig. 3
Fig. 3
Representative overview micrographs of a (a) test and (b) control methylmethacrylate sections. Arrowheads point to the apical ends of the apical root notch. Staining: toluidine blue/McNeal + basic fuchsin. GCT, gingival connective tissue; JE, junctional epithelium; NB, new bone; NC, new cementum; NPDL, new periodontal ligament
Fig. 4
Fig. 4
Histometric results comparing the test and control group
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References

    1. Wyrebek B, Gorska R, Gawron K, Gora MN, Gorski B, Plakwicz P (2021) Periodontal condition of mandibular incisors treated with modified Kazanjian vestibuloplasty compared to untreated sites: A prospective study. Adv Clin Exp Med 30(7). 10.17219/ACEM/133492 - PubMed
    1. Roccuzzo M, Bunino M, Needleman I, Sanz M. Periodontal plastic surgery for treatment of localized gingival recessions: a systematic review. J Clin Periodontol. 2002;29 Suppl 3(SUPPL. 3):178–194. doi: 10.1034/J.1600-051X.29.S3.11.X. - DOI - PubMed
    1. Chambrone L, Sukekava F, Araújo MG, Pustiglioni FE, Chambrone LA, Lima LA. Root-coverage procedures for the treatment of localized recession-type defects: a Cochrane systematic review. J Periodontol. 2010;81(4):452–478. doi: 10.1902/JOP.2010.090540. - DOI - PubMed
    1. Cheng GL, et al. Root coverage by coronally advanced flap with connective tissue graft and/or enamel matrix derivative: a meta-analysis. J Periodontal Res. 2015;50(2):220–230. doi: 10.1111/JRE.12199. - DOI - PubMed
    1. Cairo F, Pagliaro U, Nieri M. Treatment of gingival recession with coronally advanced flap procedures: a systematic review. J Clin Periodontol. 2008;35(8 Suppl):136–162. doi: 10.1111/J.1600-051X.2008.01267.X. - DOI - PubMed