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. 2023 Oct;43(10):3707-3715.
doi: 10.1007/s10792-023-02780-5. Epub 2023 Jul 8.

Effects of atorvastatin on the function of Tenon's capsule fibroblasts in human eyes

Haiying Wei  1 , Jian Wang  1 , Ruiqi Wang  1 , Yeqing Wang  1 , Xiaodan Wang  1 , Zhuolei Feng  2
Affiliations

Effects of atorvastatin on the function of Tenon's capsule fibroblasts in human eyes

Haiying Wei et al. Int Ophthalmol. 2023 Oct.

Abstract

Purpose: This study aimed to explore the role of atorvastatin (ATO) in the prevention and treatment of the scarring of filtration channels after glaucoma surgery.

Methods: Human Tenon's capsule fibroblasts (HTFs) were co-cultured with various concentrations of ATO. First, Cell Counting Kit-8 assay was performed to evaluate the effects of various concentrations of ATO on the viability of HTFs. Then, after the ATO stimulated the HTFs for 24 h, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was performed to evaluate the apoptosis of HTFs. Transwell assay was also performed to evaluate the migration of HTFs. Moreover, enzyme-linked immunosorbent assay (ELISA) was performed to detect the protein expression levels of transforming growth factor-β1 (TGF-β1) and TGF-β2 in the cell culture supernatant of HTFs. Western blot was carried out to detect the protein expression levels of smooth muscle actin (SMA), p38, Smad3, fibronectin, collagen I and collagen III in different groups.

Results: The results revealed that ATO could inhibit the proliferation and migration of HTFs. Based on the TUNEL assay, 100 μM and 150 μM ATO could induce cell apoptosis. The ELISA results indicated that ATO could down-regulate the expression level of TGF-β2, and western blot analysis revealed that the protein expression levels of SMA, p38, Smad3, fibronectin, collagen I and collagen III in the TGF-β2 group were all up-regulated compared with the control group, whereas the addition of ATO could reverse this up-regulation.

Conclusions: ATO could inhibit the proliferation and migration of HTFs and induce their apoptosis. It was preliminary proven that ATO could inhibit the signaling pathway induced by TGF-β. It is suggested that ATO could be a basis for the treatment of the scarring of filtration channels after glaucoma surgery.

Keywords: Atorvastatin; Glaucoma; Human Tenon's capsule fibroblasts; TGF-β.

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References

    1. Xing Y, Cui L, Kang Q (2016) Silencing of ILK attenuates the abnormal proliferation and migration of human Tenon’s capsule fibroblasts induced by TGF-β2. Int J Mol Med 38(2):407–416 - DOI - PubMed - PMC
    1. Gedde SJ, Singh K, Schiffman JC, Feuer WJ (2012) The tube versus trabeculectomy study: interpretation of results and application to clinical practice. Curr Opin Ophthalmol 23(2):118–126 - DOI - PubMed
    1. Fontana L, De Maria M (2021) Surgical management of pseudoexfoliative glaucoma: a review of current clinical considerations and surgical outcomes. J Glaucoma. https://doi.org/10.1097/IJG.0000000000001724 - DOI - PubMed
    1. Liu H, Zhang H, Li Y, Yu H (2017) Safety and efficacy of canaloplasty versus trabeculectomy in treatment of glaucoma. Oncotarget 8(27):44811–44818 - DOI - PubMed - PMC
    1. Grisanti S, Szurman P, Warga M, Kaczmarek R, Ziemssen F, Tatar O et al (2005) Decorin modulates wound healing in experimental glaucoma filtration surgery: a pilot study. Invest Ophthalmol Vis Sci 46(1):191–196 - DOI - PubMed

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