Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2023 Jun 30:2023:4380261.
doi: 10.1155/2023/4380261. eCollection 2023.

Evaluation of Antibiotics Residues in Milk and Meat Using Different Analytical Methods

Melaku Getahun  1 , Rahel Belete Abebe  2 , Ashenafi Kibret Sendekie  2 , Alem Endeshaw Woldeyohanis  3 , Asmamaw Emagn Kasahun  3
Affiliations
Review

Evaluation of Antibiotics Residues in Milk and Meat Using Different Analytical Methods

Melaku Getahun et al. Int J Anal Chem. .

Abstract

Veterinary drugs are pharmacologically and biologically active chemical agents. At present, veterinary drugs are extensively used to prevent and treat animal diseases, to promote animal growth, and to improve the conversion rate of feed. However, the use of veterinary drugs in food-producing animals may leave residues of the parent compounds and/or their metabolites in food products resulting in harmful effects on humans. To ensure food safety, sensitive and effective analytical methods have been developing rapidly. This review describes sample extraction and cleanup methods, and different analytical techniques are used for the determination of veterinary drug residues in milk and meat. Sample extraction methods, such as solvent extraction, liquid-liquid extraction, and cleanup methods such as dispersive solid-phase extraction and immunoaffinity chromatography, were summarized. Different types of analytical methods such as microbial, immunological, biosensor, thin layer chromatography, high-performance liquid chromatography, and liquid chromatography-tandem mass spectrometry were discussed for the analysis of veterinary drug residues in animal-derived foods. Liquid chromatography-tandem mass spectrometry is the most widely used analytical technique for the determination of antibiotic drug residues. This is due to the powerful separation of LC and accurate identification of MS, and LC-MS/MS is more popular in the analysis of veterinary drug residues.

PubMed Disclaimer

Conflict of interest statement

The authors declare that there are no conflicts of interest.

Figures

Figure 1
Figure 1
Example of typical processes for the determination of a given analyte in a meat sample.
See this image and copyright information in PMC

References

    1. Alimentarius C. Glossary of terms and definitions (residues of veterinary drugs in foods) Codex Alimentarius Commission . 2012;8:5–1993.
    1. Bion C., Beck Henzelin A., Qu Y., Pizzocri G., Bolzoni G., Buffoli E. Analysis of 27 antibiotic residues in raw cow’s milk and milk-based products–validation of Delvotest T. Food Additives and Part A, Chemistry, Analysis, Control, Exposure and Risk Assessment . 2016;33(1):54–59. doi: 10.1080/19440049.2015.1104731. - DOI - PubMed
    1. Li S., Zhang Q., Chen M., Zhang X., Liu P. Determination of veterinary drug residues in food of animal origin: sample preparation methods and analytical techniques. Journal of Liquid Chromatography & Related Technologies . 2020;43(17-18):701–724. doi: 10.1080/10826076.2020.1798247. - DOI
    1. Forouzan S., Rahimirad A., Seyedkhoei R., Asadzadeh J., Bahmani M. Determination of antibiotic residues in the pasteurized milk produced in. Journal of Coastal Life Medicine . 2014;2(4):297–301.
    1. Commission Regulation (EC) No 508/1999 of 4 March 1999 Amending Annexes I to IV to Council Regulation (EEC) No 2377/90 Laying Down a Community Procedure for the Establishment of Maximum Residue Limits of Veterinary Medicinal Products in Foodstuffs of Animal Origin . 1999.

LinkOut - more resources