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. 2023 Jun 23:13:1207319.
doi: 10.3389/fcimb.2023.1207319. eCollection 2023.

Detection of honey bee viruses in larvae of Vespa orientalis

Karen Power  1 , Manuela Martano  1 , Ernesto Ragusa  2 , Gennaro Altamura  1 , Paola Maiolino  1
Affiliations

Detection of honey bee viruses in larvae of Vespa orientalis

Karen Power et al. Front Cell Infect Microbiol. .

Abstract

The Oriental hornet (Vespa orientalis) is one of the major predators of honey bees. It has been demonstrated that adults of V. orientalis can harbor honey bee viruses, however the transmission route of infection is still not clear. The aim of this study was to study the possible presence of honey bee viruses in V. orientalis larvae and honey bees collected from the same apiary. Therefore, 29 samples of V. orientalis larvae and 2 pools of honey bee (Apis mellifera). samples were analyzed by multiplex PCR to detect the presence of six honeybee viruses: Acute Bee Paralysis Virus (ABPV), Black Queen Cell Virus (BQCV), Chronic Bee Paralysis Virus (CBPV), Deformed Wing Virus (DWV), Kashmir Bee Virus (KBV) and Sac Brood Virus (SBV). Biomolecular analysis of V. orientalis larvae revealed that DWV was present in 24/29 samples, SBV in 10/29, BQCV in 7/29 samples and ABPV in 5/29 samples, while no sample was found positive for CBPV or KBV. From biomolecular analysis of honey bee samples DWV was the most detected virus, followed by SBV, BQCV, ABPV. No honey bee sample was found positive for CBPV or KBV. Considering the overlapping of positivities between V.orientalis larvae and honey bee samples, and that V.orientalis larvae are fed insect proteins, preferably honey bees, we can suggest the acquisition of viral particles through the ingestion of infected bees. However, future studies are needed to confirm this hypothesis and rule out any other source of infection.

Keywords: ABPV; BQCV; CBPV; DWV; Oriental hornet; SBV; Vespa orientalis; honey bee.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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References

    1. Abrol D. P. (1994). Ecology, behaviour and management of social wasp, vespa velutina smith (Hym., vespidae), attacking honeybee colonies. Korean J. Apic. 9, 5–10. doi: 10.1163/187631293X00226 - DOI
    1. Archer M. E. (1998). Taxonomy, distribution and nesting biology of vespa orientalis l. Entomol. Mon. Mag. 134, 45–51.
    1. Bellucci V., Lucci S., Bianco P., Ubaldi A., Felicioli A., Porrini C., et al. . (2019). Monitoring honey bee health in five natural protected areas in Italy. Vet. Ital. 55 (1), 15–25. doi: 10.12834/VetIt.1209.6739.4 - DOI - PubMed
    1. Bertolino S., Lioy S., Laurino D., Manino A., Porporato M. (2016). Spread of the invasive yellow-legged hornet vespa velutina (Hymenoptera: vespidae) in Italy. Appl. Entomol. Zool. 51, 589–597. doi: 10.1007/s13355-016-0435-2 - DOI
    1. Bordin F., Zulian L., Granato A., Caldon M., Colamonico R., et al. . (2022). Presence of known and emerging honey bee pathogens in apiaries of veneto region (Northeast of Italy) during spring 2020 and 2021. Appl. Sci. 12, 2134. doi: 10.3390/app1204213 - DOI

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