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. 2023 Jun 23:10:1173368.
doi: 10.3389/fmed.2023.1173368. eCollection 2023.

The value of histopathology combined with CapitalBio Mycobacterium real-time polymerase chain reaction test for diagnosing spinal tuberculosis

Liwei Yao  1 , Caifang Bu  1 , Jinjuan Zhang  1 , Dandan Zhang  1   2
Affiliations

The value of histopathology combined with CapitalBio Mycobacterium real-time polymerase chain reaction test for diagnosing spinal tuberculosis

Liwei Yao et al. Front Med (Lausanne). .

Abstract

Purpose: To evaluate the diagnostic efficacy of CapitalBio Mycobacterium real-time polymerase chain reaction assay (CapitalBio test) in spinal tuberculosis (STB). The value of histopathology combined with the CapitalBio test in diagnosing STB was also assessed.

Methods: We retrospectively analyzed the medical information of suspected STB. The sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and area under the curve (AUC) of histopathology, CapitalBio test, and histopathology combined with CapitalBio test were calculated to evaluate their diagnostic efficacy compared with a composite reference standard.

Results: A total of 222 suspected STB patients were included in the study. The sensitivity, specificity, PPV, NPV, and AUC of histopathology for STB were recorded to be 62.0, 98.0, 97.4, 68.3%, and 0.80, respectively. The sensitivity, specificity, PPV, NPV, and AUC of the CapitalBio test were 75.2, 98.0, 97.9, 76.7%, and 0.87, respectively, while that of histopathology combined with the CapitalBio test was 81.0, 96.0, 96.1, 80.8%, and 0.89, respectively.

Conclusion: Histopathology and CapitalBio test exhibited high accuracy and are recommended in diagnosing STB. Histopathology combined with the CapitalBio test might give the best efficacy in STB diagnosis.

Keywords: CapitalBio test; accuracy; histopathology; molecular test; spinal tuberculosis.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Magnetic resonance imaging changes of spinal tuberculosis. (A) thoracic tuberculosis leading to destruction of vertebral body and prevertebral abscess. (B) lumbar tuberculosis leading to destruction of vertebral body, intervertebral disk and prevertebral abscess. (C) lumbar tuberculosis leading to destruction of lumbar vertebral body. (D) lumbar tuberculosis leading to worm-bite like destruction of vertebral body and intervertebral disk.
Figure 2
Figure 2
Diagnostic classification of patients included in the study.
Figure 3
Figure 3
Venn diagram of the distribution of positive patient results in different classifications. (A) All patients. (B) Spinal tuberculosis patients. (C) Non spinal tuberculosis patients.
Figure 4
Figure 4
The receiver operating characteristic curve for different tests.
See this image and copyright information in PMC

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