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. 2023 Jul 3:15:305-313.
doi: 10.2147/RRU.S406808. eCollection 2023.

High-Fat and High-Sucrose Diet Leads to Skeletal Muscle Loss and Bladder Dysfunction in Rat

Naoki Wada  1 , Noriyuki Abe  1 , Kotona Miyauchi  1 , Shogo Makino  1 , Hidehiro Kakizaki  1
Affiliations

High-Fat and High-Sucrose Diet Leads to Skeletal Muscle Loss and Bladder Dysfunction in Rat

Naoki Wada et al. Res Rep Urol. .

Abstract

Purpose: In this study, we investigated skeletal muscle loss and bladder dysfunction caused by high-fat/high-sucrose (HFS) diet.

Methods: Twelve-week-old Sprague-Dawley (SD) female rats were fed on normal (Group N) or HFS (Group HFS) diet for 12 weeks. We conducted urodynamic investigation and pharmacologic in vitro. In addition, we measured gastrocnemius and tibialis muscle weight and protein concentration. The hypoxia-inducible factor (HIF)-1α and 8-hydroxy-2'-deoxyguanosine (8-OHdG) in the bladder were assayed.

Results: The urodynamic investigations revealed the significantly shorter intercontraction intervals and lower maximal voiding pressure in Group HFS than in Group N. Furthermore, the absolute and relative weights of the gastrocnemius muscle were found to be significantly lower in Group HFS than in Group N. The protein concentration of the gastrocnemius muscle was also significantly lower in Group HFS than in Group N. The absolute and relative weights of the bladder were also significantly lower in Group HFS than in Group N. The contractile responses of the bladder strips to electrical field stimulation and carbachol were significantly lower in Group HFS than in Group N. The HIF1α and 8OHdG in the bladder muscle were significantly higher in Group HFS than in Group N. The HFS diet reduced bladder capacity and contractility along with the loss of the gastrocnemius muscle.

Conclusion: HFS diet promotes bladder dysfunction similar to detrusor hyperreflexia with impaired contractility.

Keywords: bladder dysfunction; high-fat; high-sucrose; rat; skeletal muscle.

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Conflict of interest statement

All authors do not have any conflict of interest to disclose.

Figures

Figure 1
Figure 1
Representative cystometry traces in a rat fed on normal diet (A) and HFS diet (B). Compared with normal diet rat, the intercontraction interval was shorter and voiding pressure lower in HFS diet rat.
Figure 2
Figure 2
Analyses of the urodynamic parameters in rats fed on normal and HFS diets. N = 10 in Group N and N = 9 in Group HFS. The inter-contraction interval was significantly shorter in HFS diet rats than normal diet rats. The maximal voiding pressure was significantly lower in HFS diet rats. The voided volume and bladder capacity were significantly smaller in HFS diet rats. The post-void residual and voiding efficiency were similar between the 2 groups. *P < 0.05 using Mann–Whitney U-test.
Figure 3
Figure 3
Maximal contraction of bladder strips normalized to strip weight (A) and the response to KCl (B) exposed to electrical field stimulation (2, 8 and 32 Hz at 20 volts), carbachol (20 µM), and KCl (120 mM) in normal and HFS diet groups. N = 8 in Group N and N = 6 in Group HFS. *P < 0.05 using Mann–Whitney U-test.
Figure 4
Figure 4
The protein concentration of the tibialis and gastrocnemius muscle. The protein concentration of the gastrocnemius muscle in Group HFS was significantly lower than that in Group N. *P < 0.05 using Mann–Whitney U-test.
Figure 5
Figure 5
Bladder HIF1α (A) and 8OHdG (B) protein assay. HIF1α and 8OHdG in bladder muscle were significantly increased in HFS diet rats than in normal diet rats. N = 18 in Group N and N = 15 in Group HFS. *P < 0.05 using Mann–Whitney U-test.
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