The membrane phenotype of in vivo induced tumor selective cytolytic lymphocytes of the rat: a distinction from NK cells

Abstract

The phenotype of tumor selective cytolytic lymphocytes of the rat is defined by staining of peritoneal cells of tumor-immunized donors with the monoclonal antibodies OX19, OX8, and W3/25, sorting in a cytofluorometer and evaluating cytolytic capacity in a 51Cr release assay. It is shown that the tumor selective cytolytic lymphocytes bear the OX19 and OX8 markers but are lacking the W3/25 marker. It is also shown that the OX19+ lymphocytes do not contain any NK-like activity. The OX19 marker can therefore distinguish cells that execute selective cytolysis from cells with NK-like activities.