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Published Erratum
. 2023 Jul 18;120(29):e2310153120.
doi: 10.1073/pnas.2310153120. Epub 2023 Jul 10.

Correction for Li et al., SARS-CoV-2 induces double-stranded RNA-mediated innate immune responses in respiratory epithelial-derived cells and cardiomyocytes

No authors listed
Published Erratum

Correction for Li et al., SARS-CoV-2 induces double-stranded RNA-mediated innate immune responses in respiratory epithelial-derived cells and cardiomyocytes

No authors listed. Proc Natl Acad Sci U S A. .
No abstract available

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Figures

Fig. 2.
Fig. 2.
Infection of nasal epithelia-derived cells by SARS-CoV-2 and MERS-CoV. Nasal cells, cultured in air–liquid transwells, were mock-infected or infected apically with SARS-CoV-2 (multiplicity of infection, MOI = 5) and in (A) MERS-CoV (MOI = 5). (A) At indicated times, apically released virus was quantified by plaque assay on Vero-E6 cells. Values are means ± SD (error bars). Statistical significance (not displayed) was determined by two-way ANOVA (*P < 0.05). One experiment was performed using four separate donors. (B) At 48 hpi, nasal cells were fixed and permeabilized. N protein (red) of SARS-CoV-2 and MERS-CoV was detected with an anti-N antibody, and cilia (green) detected with an anti-type IV β-tubulin antibody by immunofluorescence assay (IFA). One representative image is shown from at least three independent experiments, with four donors for each virus infection. (Scale bars, 100 μm.) (C) At 120 hpi, cells were lysed, and proteins were analyzed by immunoblotting with antibodies as indicated. One experiment using three separate donors was performed. Cells from a fourth donor (#13) were mock-treated or treated with IFN-α (500 Units/mL) for 1 h before lysis and protein lysates from Calu-3 cells (mock or SARS-CoV-2; MOI = 5); infected Calu-3 cells 24 hpi were also analyzed. (D) At 120 hpi, total RNA was harvested, and mRNA expression level quantified by qRT-PCR. CT values were normalized to 18S rRNA and expressed as fold-change over mock displayed as 2−Δ(ΔCt). Technical replicates were averaged, the means for each replicate displayed, ±SD. One experiment was performed using three separate donor (#9, #10, #11) samples. (E) RNA was harvested from two donors at 120 hpi and rRNA integrity determined by Bioanalyzer. The position of 28S and 18S rRNA are indicated. Data shown are from one representative experiment of two independent experiments (SI Appendix, Figs. S1A and S2).
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