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Comparative Study
. 1986;3(1):25-39.
doi: 10.1159/000467875.

A sensitive ELISA for the quantitation of human C5a in blood plasma using a monoclonal antibody

M Schulze, O Götze
Comparative Study

A sensitive ELISA for the quantitation of human C5a in blood plasma using a monoclonal antibody

M Schulze et al. Complement. 1986.

Abstract

An ELISA for the quantitation of the C5a anaphylatoxin of complement in human plasma is presented which is based on the use of both a monoclonal antibody and polyclonal antibodies to C5a. Its detection limit is 1 ng C5a/ml plasma. It detects no C5a in fresh EDTA-plasma. Complete removal of C5 by an optimized precipitation step prior to the assay procedure is an essential feature of the method.

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