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. 2023 Jul 11;23(1):355.
doi: 10.1186/s12870-023-04329-1.

Genetic fingerprint construction and genetic diversity analysis of sweet potato (Ipomoea batatas) germplasm resources

Zhongxia Luo  1 , Zhufang Yao  1 , Yiling Yang  1 , Zhangying Wang  1 , Hongda Zou  1 , Xiongjian Zhang  1 , Jingyi Chen  1 , Boping Fang  2 , Lifei Huang  3
Affiliations

Genetic fingerprint construction and genetic diversity analysis of sweet potato (Ipomoea batatas) germplasm resources

Zhongxia Luo et al. BMC Plant Biol. .

Abstract

Background: China is the largest producer of sweet potato in the world, accounting for 57.0% of the global output. Germplasm resources are the basis for promoting innovations in the seed industry and ensuring food security. Individual and accurate identification of sweet potato germplasm is an important part of conservation and efficient utilization.

Results: In this study, nine pairs of simple sequence repeat molecular markers and 16 morphological markers were used to construct genetic fingerprints for sweet potato individual identification. Combined with basic information, typical phenotypic photographs, genotype peak graphs, and a two-dimensional code for detection and identification were generated. Finally, a genetic fingerprint database containing 1021 sweet potato germplasm resources in the "National Germplasm Guangzhou Sweet Potato Nursery Genebank in China" was constructed. Genetic diversity analysis of the 1021 sweet potato genotypes using the nine pairs of simple sequence repeat markers revealed a narrow genetic variation range of Chinese native sweet potato germplasm resources, and Chinese germplasm was close to that from Japan and the United States, far from that from the Philippines and Thailand, and the furthest from that from Peru. Sweet potato germplasm resources from Peru had the richest genetic diversity, supporting the view that Peru is the center of origin and domestication of sweet potato varieties.

Conclusions: Overall, this study provides scientific guidance for the conservation, identification, and utilization of sweet potato germplasm resources and offers a reference to facilitate the discovery of important genes to boost sweet potato breeding.

Keywords: Genetic diversity; Genetic fingerprint; Genetic markers; Germplasm resources; Sweet potato.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Kaiser-Meyer-Olkin measures and selection of morphological markers
Fig. 2
Fig. 2
Electrophoresis and selection of core simple sequence repeat (SSR) primers. a Electrophoretic gel of primers 1–12, (b) Electrophoretic gel of primers 13–23. 1–23 blace numbers: SSR primers, 1–4 red numbers: sweet potato resources
Fig. 3
Fig. 3
Average genetic distances in sweet potato populations by country
Fig. 4
Fig. 4
Variation trends in different genetic distance ranges for sweet potato germplasm
Fig. 5
Fig. 5
Unweighted pair group method with arithmetic mean dendrogram of sweet potatoes. For the analysis, 1076 sweet potatoes (1021 samples from natural populations and 55 from sib populations) were considered based on the SSR markers
Fig. 6
Fig. 6
Principal component analysis of 1021 accessions based on 9 pairs SSR markers
Fig. 7
Fig. 7
Phenotypic codes and pictures for sweet potato germplasm
Fig. 8
Fig. 8
Two-dimensional code of the sweet potato genotype Guangshu 87
See this image and copyright information in PMC

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