Olfactory-driven beta band entrainment of limbic circuitry during neonatal development

Abstract

Cognitive processing relies on the functional refinement of the limbic circuitry during the first two weeks of life. During this developmental period, when the auditory, somatosensory and visual systems are still largely immature, the sense of olfaction acts as 'door to the world', providing an important source of environmental inputs. However, it is unknown whether early olfactory processing shapes the activity in the limbic circuitry during neonatal development. Here, we address this question by combining simultaneous in vivo recordings from the olfactory bulb (OB), lateral entorhinal cortex (LEC), hippocampus (HP) and prefrontal cortex (PFC) with olfactory stimulation as well as opto- and chemogenetic manipulations of mitral/tufted cells in the OB of non-anaesthetized neonatal mice of both sexes. We show that the neonatal OB synchronizes the limbic circuity in the beta frequency range. Moreover, it drives neuronal and network activity in LEC, as well as subsequently, HP and PFC via long-range projections from mitral cells to HP-projecting LEC neurons. Thus, OB activity shapes the communication within limbic circuits during neonatal development. KEY POINTS: During early postnatal development, oscillatory activity in the olfactory bulb synchronizes the limbic circuit. Olfactory stimulation boosts firing and beta synchronization along the olfactory bulb-lateral entorhinal cortex-hippocampal-prefrontal pathway. Mitral cells drive neuronal and network activity in the lateral entorhinal cortex (LEC), as well as subsequently, the hippocampus (HP) and the prefrontal cortex (PFC) via long-range projections from mitral cells to HP-projecting LEC neurons. Inhibition of vesicle release on LEC targeting mitral cell axons reveals direct involvement of LEC in the olfactory bulb-driven oscillatory entrainment of the limbic circuitry.

Keywords: beta oscillations; development; entorhinal cortex; hippocampus; olfaction; olfactory bulb; prefrontal cortex.