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. 1986 Jul;5(7):1633-44.
doi: 10.1002/j.1460-2075.1986.tb04406.x.

Adenoviral protein VII packages intracellular viral DNA throughout the early phase of infection

Adenoviral protein VII packages intracellular viral DNA throughout the early phase of infection

P K Chatterjee et al. EMBO J. 1986 Jul.

Abstract

The proteins associated with parental, adenoviral DNA in productively-infected HeLa cells have been examined both directly and indirectly. HeLa cells infected with 32P-labelled Ad2 were irradiated with u.v. light at various points in the infectious cycle. Following degradation of the DNA, nuclear proteins carrying cross-linked nucleotides, or oligonucleotides, were distinguished from virion phosphoproteins by the resistance of their 32P radioactivity to 1 M NaOH. The major core protein of the virion, protein VII, was found to be associated with viral DNA throughout infection, even when cells were infected at a multiplicity of 0.14. Micrococcal nuclease digestion of intranuclear viral DNA 4 h after infection liberated two nucleoprotein particles containing viral DNA, neither of which co-migrated with HeLa cell mononucleosomes. These results indicate that core protein VII remains associated with parental adenoviral DNA during productive infections. The observation that protein VII can be cross-linked to DNA in cells infected at very low multiplicity, together with the results of a comparison of proteins cross-linkable to viral DNA in cells infected by wild-type virus and a non-infectious mutant containing the precursor to protein VII, suggest that nucleoproteins comprising viral DNA and protein VII must be the templates for expression of pre-early and early viral genes.

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References

    1. Cell. 1978 Dec;15(4):1463-75 - PubMed
    1. Cell. 1980 Dec;22(3):905-16 - PubMed
    1. J Biol Chem. 1977 Feb 10;252(3):1102-6 - PubMed
    1. J Virol. 1979 Oct;32(1):91-7 - PubMed
    1. Biochemistry. 1973 Jul 31;12(16):3055-63 - PubMed

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