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. 2023:2685:35-51.
doi: 10.1007/978-1-0716-3295-6_3.

Manual Silica-Based DNA Extractions

Catherine Cupples Connon  1
Affiliations

Manual Silica-Based DNA Extractions

Catherine Cupples Connon. Methods Mol Biol. 2023.

Abstract

There are several silica-based extraction methods that utilize silica-packed columns or silica-coated paramagnetic resin and are suitable for the needs of forensic DNA analysis and/or human identification. These rely on the use of chaotropic salts to alter the affinity of DNA such that it binds strongly to silica. A variety of samples can be successfully processed with these procedures, including buccal swabs, dried or liquid blood, saliva, semen, and other typical forensic-type samples. This chapter will describe the manual extraction process for Promega's DNA™ IQ System, as well as Qiagen's QIAamp® DNA Blood Mini Kit, QIAamp® DNA Mini Kit, and QIAamp® DNA Investigator Kit.

Keywords: DNA IQ System; DNA extraction; Paramagnetic resin; QIAamp DNA Blood Mini Kit; QIAamp DNA Investigator Kit; QIAamp DNA Mini Kit; Silica.

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References

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