Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2023:1415:565-569.
doi: 10.1007/978-3-031-27681-1_82.

Primary Retinal Cell Cultures as a Model to Study Retina Biology

Germán A Michelis  1   2 , Luis E Politi  2 , S Patricia Becerra  3
Affiliations
Review

Primary Retinal Cell Cultures as a Model to Study Retina Biology

Germán A Michelis et al. Adv Exp Med Biol. 2023.

Abstract

Since its inception, primary retinal cultures have been an in vitro tool for modeling the in vivo environment of the retina for biological studies on development and disease. They offer simple and controlled experimental approaches when compared to in vivo models. In this review we highlight the strengths and weaknesses of primary retinal culture models, and the features of dispersed retinal cell cultures.

Keywords: Cell lines; In vitro models; In vivo models; Organoids; Primary cultures; Retina.

PubMed Disclaimer

References

    1. Agranoff BW, Feldman EL, Heacock AM, Schwartz M. The retina as a biochemical model of central nervous system regeneration. Neurochem Int. 1980;1C:487–500. - PubMed
    1. Ames A, Nesbett FB. In vitro retina as an experimental model of the central nervous system. J Neurochem. 1981;37:867–77. - PubMed
    1. Bullock J, Pagan-Mercado G, Becerra SP. Cell-based assays to identify novel retinoprotective agents. MethodsX. 2020;7:101026. - PubMed - PMC
    1. Dunn KC, Aotaki-Keen AE, Putkey FR, Hjelmeland LM. ARPE-19, a human retinal pigment epithelial cell line with differentiated properties. Exp Eye Res. 1996;62:155–69. - PubMed
    1. Fischer RA, Zhang Y, Risner ML, Li D, Xu Y, Sappington RM. Impact of graphene on the efficacy of neuron culture substrates. Adv Healthc Mater. 2018;7:e1701290. - PubMed - PMC

LinkOut - more resources