Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Nov;79(11):4626-4634.
doi: 10.1002/ps.7664. Epub 2023 Jul 23.

Novel mandelic acid derivatives suppress virulence of Ralstonia solanacearum via type III secretion system

Qiao-Qiao Guo  1 , Yu-Zhen Li  1 , Hua-Bin Shi  2 , Ao-Yun Yi  1 , Xiao-Li Xu  3 , Hai-Hong Wang  4 , Xin Deng  5   6 , Zhi-Bing Wu  2 , Zi-Ning Cui  1
Affiliations

Novel mandelic acid derivatives suppress virulence of Ralstonia solanacearum via type III secretion system

Qiao-Qiao Guo et al. Pest Manag Sci. 2023 Nov.

Abstract

Background: Bacterial wilt induced by Ralstonia solanacearum is regarded as one of the most devastating diseases. However, excessive and repeated use of the same bactericides has resulted in development of bacterial resistance. Targeting bacterial virulence factors, such as type III secretion system (T3SS), without inhibiting bacterial growth is a possible assay to discover new antimicrobial agents.

Results: In this work, identifying new T3SS inhibitors, a series of mandelic acid derivatives with 2-mercapto-1,3,4-thiazole moiety was synthesized. One of them, F-24, inhibited the transcription of hrpY gene significantly. The presence of this compound obviously attenuated hypersensitive response (HR) without inhibiting bacterial growth of R. solanacearum. The transcription levels of those typical T3SS genes were reduced to various degrees. The test of the ability of F-24 in protecting plants demonstrated that F-24 protected tomato plants against bacterial wilt without restricting the multiplication of R. solanacearum. The mechanism of this T3SS inhibition is through the PhcR-PhcA-PrhG-HrpB pathway.

Conculsion: The screened F-24 could inhibit R. solanacearum T3SS and showed better inhibitory activity than previously reported inhibitors without affecting the growth of the strain, and F-24 is a compound with good potential in the control of R. solanacearum. © 2023 Society of Chemical Industry.

Keywords: Ralstonia solanacearum; bacterial wilt; inhibitor; mandelic acid derivatives; mechanism; type III secretion system (T3SS).

PubMed Disclaimer

References

REFERENCES

    1. Genin S and Denny TP, Pathogenomics of the Ralstonia solanacearum species complex. Annu Rev Phytopathol 50:67-89 (2012).
    1. Mansfield J, Genin S, Magori S, Citovsky V, Sriariyanum M, Ronald P et al., Top 10 plant pathogenic bacteria in molecular plant pathology. Mol Plant Pathol 13:614-629 (2012).
    1. Wicker E, Grassart L, Coranson-Beaudu R, Mian D, Guilbaud C, Fegan M et al., Ralstonia solanacearum strains from Martinique (French West Indies) exhibiting a new pathogenic potential. Appl Environ Microbiol 73:6790-6801 (2007).
    1. Schell MA, Control of virulence and pathogenicity genes of Ralstonia solanacearum by an elaborate sensory network. Annu Rev Phytopathol 38:263-292 (2000).
    1. Vasse J, Frey P and Trigalet A, Microscopic studies of intercellular infection and protoxylem invasion of tomato roots by pseudomonas solanacearum. Mol Plant Microbe Interact 8:241-251 (1995).

LinkOut - more resources