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. 2023 Jul 13;55(4):267.
doi: 10.1007/s11250-023-03657-6.

Mitigation of endogenous oxidative stress and improving growth, hemato-biochemical parameters, and reproductive performance of Zaraibi goat bucks by dietary supplementation with Chlorella vulgaris or/and vitamin C

A E Abdel-Khalek  1 , M M El-Maghraby  2 , Zizy I Elbialy  3 , Rasha A Al Wakeel  4 , E A Almadaly  5 , M Shukry  4 , A A El-Badawy  2 , H K Zaghloul  6 , Doaa H Assar  7
Affiliations

Mitigation of endogenous oxidative stress and improving growth, hemato-biochemical parameters, and reproductive performance of Zaraibi goat bucks by dietary supplementation with Chlorella vulgaris or/and vitamin C

A E Abdel-Khalek et al. Trop Anim Health Prod. .

Abstract

This study was conducted to explore the effects of dietary inclusion of Chlorella vulgaris (CV) or/and vitamin C (VC) on growth, hemato-biochemical parameters, oxidative and antioxidant status, reproductive hormones, and semen quality variables, and scrotal-testicular dimensions of Zaraibi goat bucks. Twenty sexually mature bucks (41.49 ± 0.91 kg BW) were randomly divided into 4 groups (5 bucks/group). The control group was fed the control diet, while the other three groups received a diet supplemented with VC (2 g/animal /day), CV (5 g/animal/day), and CV plus VC (the same levels), respectively, for 8 weeks (treatment period), and then semen was collected for 8 weeks. Results showed that dietary supplementation with CV-VC combination significantly increased the final body weight, weight gain, packed cell volume, hemoglobin, red blood cells, white blood cells, and lymphocytes; elevated serum total protein, globulin, testosterone, estradiol, superoxide dismutase, glutathione peroxidase with a significant reduction in Malondialdehyde in serum and seminal plasma. Also, the CV-VC combination significantly improved the ejaculate volume, total sperm output, sperm concentration, and live sperm, and reduced reaction time and sperm abnormality of bucks. Either CV or VC given separately or in combination, at the chosen levels, had no detrimental effects on animal physiological responses with normal hepatic and renal functions. Therefore, the CV-VC combination could be safely utilized as a dietary supplement in buck's diets to improve antioxidant defenses, scavenge free radicals, and potentiate buck's reproductive activities under normal conditions.

Keywords: Goat; Lipid peroxidation; Microalgae; Semen; Vitamin C.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Fig. 1
Fig. 1
Hematological findings included red blood cells (RBCs), hemoglobin (Hb), packed cell volume (PCV), and white blood cells (WBCs) of bucks in the experimental groups. Data are expressed as mean ± SE (n = 5). *Significant differences at P < 0.05, **significantly differences at P < 0.01
Fig. 2
Fig. 2
Level of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in blood serum of bucks in the experimental groups. Data are expressed as mean ± SE (n = 5). *Significant differences at P < 0.05, **significant differences at P < 0.01, ***significant differences at P < 0.001, ****significant differences at P < 0.0001
Fig. 3
Fig. 3
Level of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in the seminal plasma of bucks in the experimental groups. Data are expressed as mean ± SE (n = 5). *Significant differences at P < 0.05
Fig. 4
Fig. 4
The concentration of testosterone in blood serum and semen, and estradiol (E2) in the semen of bucks in the experimental groups. Data are expressed as mean ± SE (n = 5). *Significant differences at P < 0.05, **significant differences at P < 0.01, ***significant differences at P < 0.001
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