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. 2023 Jun 21;13(13):2056.
doi: 10.3390/ani13132056.

Serological Examinations of Significant Viral Infections in Domestic Donkeys at the Special Nature Reserve "Zasavica", Serbia

Sava Lazić  1 , Sara Savić  1 , Tamaš Petrović  1 , Gospava Lazić  1 , Marina Žekić  1 , Darko Drobnjak  2 , Diana Lupulović  1
Affiliations

Serological Examinations of Significant Viral Infections in Domestic Donkeys at the Special Nature Reserve "Zasavica", Serbia

Sava Lazić et al. Animals (Basel). .

Abstract

The paper presents the findings of specific antibodies in the blood sera of donkeys against the following viruses: equine infectious anemia virus (EIAV), African horse sickness virus (AHSV), equine herpesvirus type 1 (EHV-1), equine influenza virus subtype H3N8 (EIV) and equine arteritis virus (EAV). The analyses were conducted during the year 2022. From a total of 199 donkeys bred in "Zasavica", blood was sampled from 53 animals (2 male donkeys and 51 female donkeys), aged 3 to 10 years. Specific antibodies against EIAV were not detected in any of the tested animals using the agar-gel immunodiffusion (AGID) assay. No specific antibodies against AHSV, tested by enzyme-linked immunosorbent assay (ELISA), or antibodies against EAV, tested by the virus neutralization test (VNT) and ELISA were detected in any of these animals. A positive serological result for EHV-1 was determined by the VNT in all animals, with antibody titer values ranging from 1:2 to 1:128, while a very low antibody titer value for EIV (subtype H3N8) of 1:16 was determined in 18 donkeys using the hemagglutination inhibition test (HI test).

Keywords: African horse sickness; Serbia; domestic donkey; equine herpesvirus type 1 infection; equine infectious anemia; equine influenza; equine viral arteritis.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Location of the Special Nature Reserve “Zasavica”, Republic of Serbia.
Figure 2
Figure 2
Age categories of 53 randomly selected donkeys in the Special Nature Reserve “Zasavica”, Republic of Serbia.
Figure 3
Figure 3
Comparative presentation of antibodies against EHV-1 and EIV in donkeys in relation to the age of the animals.
See this image and copyright information in PMC

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