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. 2023 Jun 28;15(13):2856.
doi: 10.3390/polym15132856.

Influence of Reduction with NaBH4 and HCl in Obtaining Amino Derivatives of Cashew Gum and Cytotoxic Profile

Francisco das C M Brito  1   2   3 , Wilton C Lopes  2   4 , Fábio O S Ribeiro  2 , Raiza Raianne Luz Rodrigues  5 , Klinger Antonio da França Rodrigues  5 , Fabrício Dos Santos Machado  6 , Ana Jérsia Araújo  6 , José Delano Barreto Marinho Filho  6 , Antônia Carla J Oliveira  7 , Edson C S Filho  7 , Irisvan S Ribeiro  8 , Regina C M de Paula  8 , Fernando Hallwass  9 , Vicente Gálber F Viana  3 , Durcilene A Silva  2
Affiliations

Influence of Reduction with NaBH4 and HCl in Obtaining Amino Derivatives of Cashew Gum and Cytotoxic Profile

Francisco das C M Brito et al. Polymers (Basel). .

Abstract

Tree-exuded gums are natural polymers that represent an abundant raw material in the food and pharmaceutical industries. The cashew gum can be obtained by exudation of trees of the genus Anacardium, a native species of the Brazilian northeast; its polymer consists of monosaccharide units propitious to the action of chemical reactions that tend to improve their intrinsic characteristics among them, as the degree of hydro-solubility. The objective of this work was to modify the exudate gum of Anacardium occidentale (cashew gum (CG)) through an amine reaction. The modification was confirmed by Nuclear Magnetic Resonance (1H NMR), infrared spectroscopy (FTIR), gel permeation chromatography (GPC), zeta potential, and thermogravimetric analysis (TG). In addition, the chemical modification altered the molar mass and surface charge of the CG, and the amino group binding to the CG polymers was confirmed by FTIR spectra. In addition, cytotoxicity tests were performed where cell viability was estimated by an MTT assay on RAW 264.7 macrophages. Through these tests, it was found that the amine caused an increase in the thermal stability of the amino compounds and did not present cytotoxic potential at concentrations below 50.0 mg/L.

Keywords: amin; cashew gum; modification; polysaccharide.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Mechanism of Cashew Gum Amination.
Figure 2
Figure 2
Spectrum of ftir CG, CGA1 and CGA2.
Figure 3
Figure 3
Thermogravimetric curves Tg (A) and dTG (B) for cashew gum and amine derivatives in N2 atmosphere at a heating rate of 10 °C/min. (I, II, III and IV temperatures at which events occur).
Figure 4
Figure 4
Mass distribution in the GPC.
Figure 5
Figure 5
NMR spectra CG(A), ACG1 (B), and ACG2 (C).
Figure 6
Figure 6
Cytotoxicity on macrophages RAW 264.7 and evaluation of the cytotoxic activity in vitro of modified cashew gum against HCT-116, MDA-MB-231, and L929 strains, after 72 h of incubation determined by the MTT method. ((*) concentration of the amine derivative used in the test).
See this image and copyright information in PMC

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