Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2023 Jun 28:14:1209879.
doi: 10.3389/fimmu.2023.1209879. eCollection 2023.

Advances in genetic manipulation of Chlamydia trachomatis

Weiqiang Wan  1 , Danni Li  1 , Dan Li  2 , Jun Jiao  1
Affiliations
Review

Advances in genetic manipulation of Chlamydia trachomatis

Weiqiang Wan et al. Front Immunol. .

Abstract

Chlamydia trachomatis, one species of Chlamydia spp., has the greatest impact on human health and is the main cause of bacterial sexually transmitted diseases and preventable blindness among all Chamydia spp. species. The obligate intracellular parasitism and unique biphasic developmental cycle of C. trachomatis are the main barriers for the development of tools of genetic manipulation. The past decade has witnessed significant gains in genetic manipulation of C. trachomatis, including chemical mutagenesis, group II intron-based targeted gene knockout, fluorescence-reported allelic exchange mutagenesis (FRAEM), CRISPR interference (CRISPRi) and the recently developed transposon mutagenesis. In this review, we discuss the current status of genetic manipulations of C. trachomatis and highlights new challenges in the nascent field of Chlamydia genetics.

Keywords: Chlamydia trachomatis; challenges; genetic manipulation; intracellular bacterium; transformation.

PubMed Disclaimer

Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Progressing of the genetic manipulation of Chlamydia trachomatis. FRAEM, fluorescence-reported allelic exchange mutagenesis. CRISPRi, CRISPR interference.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Elwell C, Mirrashidi K, Engel J. Chlamydia cell biology and pathogenesis. Nat Rev Microbiol (2016) 14:385–400. doi: 10.1038/nrmicro.2016.30 - DOI - PMC - PubMed
    1. Solomon AW, Burton MJ, Gower EW, Harding-Esch EM, Oldenburg CE, Taylor HR, et al. . Trachoma. Nat Rev Dis Primers (2022) 8:32. doi: 10.1038/s41572-022-00359-5 - DOI - PubMed
    1. Lujan AL, Croci DO, Gambarte Tudela JA, Losinno AD, Cagnoni AJ, Marino KV, et al. . Glycosylation-dependent galectin-receptor interactions promote Chlamydia trachomatis infection. Proc Natl Acad Sci USA (2018) 115:E6000–9. doi: 10.1073/pnas.1802188115 - DOI - PMC - PubMed
    1. Sixt BS, Valdivia RH. Molecular genetic analysis of Chlamydia species. Annu Rev Microbiol (2016) 70:179–98. doi: 10.1146/annurev-micro-102215-095539 - DOI - PubMed
    1. Moore ER, Ouellette SP. Reconceptualizing the chlamydial inclusion as a pathogen-specified parasitic organelle: an expanded role for inc proteins. Front Cell Infect Microbiol (2014) 4:157. doi: 10.3389/fcimb.2014.00157 - DOI - PMC - PubMed

Publication types