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. 2023 Jul 14;72(3):383-392.
doi: 10.33549/physiolres.935091.

The effects of subtoxic dose of acetaminophen combined with exercise on the liver of rats

I Aksu  1 , M Kiray, C Gencoglu, A Tas, O Acikgoz
Affiliations

The effects of subtoxic dose of acetaminophen combined with exercise on the liver of rats

I Aksu et al. Physiol Res. .

Abstract

Regular physical exercise is beneficial to the body. Acute exercise causes oxidant stress in many tissues including the liver by creating an unbalanced status between oxidant and antioxidant levels. Analgesic drugs are commonly consumed to reduce the pain after exercise. Acetaminophen (APAP), commonly used as an over-the-counter analgesic, can cause hepatotoxicity. The aim of this study was to investigate the effect and underlying mechanisms of APAP at subtoxic dose, which is given after the acute and exhaustive exercise on the rat livers. Male Wistar rats weighing 200-250 g were divided into 6 groups each consisting of 7 rats/group; Control, APAP (250 mg/kg, ip), Acute Exercise (AEx), Acute Exhaustive Exercise (AEEx), Acute Exercise and APAP (AEx+APAP) and Acute Exhaustive Exercise and APAP (AEEx+APAP) groups. Rats were exercised at moderate intensity or exhaustive on the treadmill and then received APAP. Tissue MDA levels were significantly increased in AEEx, AEx+APAP and AEEx+APAP groups compared with the control. There was no significant difference in GSH levels between groups. Tissue Sirtuin1 (Sirt1) levels of APAP, AEx and AEEx groups were significantly less than control. There was no significant difference between groups in VEGF levels. Liver damage score was significantly higher in all groups compared with control group. As a result, this study shows that subtoxic dose of APAP treatment alone or in combination with acute or exhaustive treadmill exercise can cause oxidative liver damage by affecting Sirt1 levels and without affecting VEGF levels.

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Conflict of interest statement

Conflict of Interest

There is no conflict of interest.

Figures

Fig. 1
Fig. 1
Representative photomicrographs of H&E staining. Stars indicate congestion, black arrows; inflammatory cell infiltration, N; necrotic areas, bar=20 μm.
Fig. 2
Fig. 2
Representative photomicrographs of Masson trichrome staining. Arrows; collagen fibers, bar=20 μm.
See this image and copyright information in PMC

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References

    1. Donaldson LJ. Sport and exercise: the public health challenge. Br J Sports Med. 2000;34:409–410. doi: 10.1136/bjsm.34.6.409. - DOI - PMC - PubMed
    1. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007;39:44–84. doi: 10.1016/j.biocel.2006.07.001. - DOI - PubMed
    1. Banerjee AK, Mandal A, Chanda D, Chakraborti S. Oxidant, antioxidant and physical exercise. Mol Cell Biochem. 2003;253:307–312. doi: 10.1023/A:1026032404105. - DOI - PubMed
    1. Lew H, Quintanilha A. Effects of endurance training and exercise on tissue antioxidative capacity and acetaminophen detoxification. Eur J Drug Metab Pharmacokinet. 1991;16:59–68. doi: 10.1007/BF03189876. - DOI - PubMed
    1. Ji LL, Fu R. Responses of glutathione system and antioxidant enzymes to exhaustive exercise and hydroperoxide. J Appl Physiol (1985) 1992;72:549–554. doi: 10.1152/jappl.1992.72.2.549. - DOI - PubMed