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. 2023:2690:311-334.
doi: 10.1007/978-1-0716-3327-4_26.

Cataloguing Protein Complexes In Planta Using TurboID-Catalyzed Proximity Labeling

Lore Gryffroy  1   2   3 , Joren De Ryck  1   2   3 , Veronique Jonckheere  3 , Sofie Goormachtig  1   2 , Alain Goossens  1   2 , Petra Van Damme  4
Affiliations

Cataloguing Protein Complexes In Planta Using TurboID-Catalyzed Proximity Labeling

Lore Gryffroy et al. Methods Mol Biol. 2023.

Abstract

Mapping protein-protein interactions is crucial to understand protein function. Recent advances in proximity-dependent biotinylation (BioID) coupled to mass spectrometry (MS) allow the characterization of protein complexes in diverse plant models. Here, we describe the use of BioID in hairy root cultures of tomato and provide detailed information on how to analyze the data obtained by MS.

Keywords: Interactomics; Mass spectrometry; Protein; Protein interactions; Proximity-dependent biotinylation; Solanaceae hairy root cultures; TurboID.

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