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Review
. 2024 Apr;196(4):1769-1785.
doi: 10.1007/s12010-023-04643-2. Epub 2023 Jul 15.

Arylacetonitrilases: Potential Biocatalysts for Green Chemistry

Tek Chand Bhalla  1 , Neerja Thakur  2   3 , Vijay Kumar  2   4
Affiliations
Review

Arylacetonitrilases: Potential Biocatalysts for Green Chemistry

Tek Chand Bhalla et al. Appl Biochem Biotechnol. 2024 Apr.

Abstract

Nitrilases are the enzymes that catalyze the hydrolysis of nitriles to corresponding carboxylic acid and ammonia. They are broadly categorized into aromatic, aliphatic, and arylacetonitrilases based on their substrate specificity. Most of the studies pertaining to these enzymes in the literature have focused on aromatic and aliphatic nitrilases. However, arylacetonitrilases have attracted the attention of academia and industry in the last several years due to their aryl specificity and enantioselectivity. They have emerged as interesting biocatalytic tools in green chemistry to synthesize useful aryl acids such as mandelic acid and derivatives of phenylacetic acid. The aim of the present review is to collate information on the arylacetonitrilases and their catalytic properties including enantioselectivity and potential applications in organic synthesis.

Keywords: Arylacetic acids; Arylacetonitrilase; Biocatalyst; Enantioselectivity; Nitrilase.

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References

    1. Thimann, K. V., & Mahadevan, S. (1964). Nitrilase I. Occurrence, preparation, and general properties of the enzyme. Archieves of Biochemistry and Biophysics, 105, 133–141. - DOI
    1. Mahadevan, S., & Thimann, K. V. (1964). Nitrilase II. Substrate specificity and possible mode of action. Archieves of Biochemistry and Biophysics, 107, 62–68. - DOI
    1. Bhalla, T. C., Kumar, V., & Kumar, V. (2018). Enzymes of aldoxime–nitrile pathway for organic synthesis. Reviews in Environmental Science and Biotechnology, 17, 229–239. - DOI
    1. Bhalla, T. C., Kumar, V., Kumar, V., & ThakurSavitri, N. (2018). Nitrile metabolizing enzymes in biocatalysis and biotransformation. Applied Biochemistry and Biotechnology, 185, 925–946. - PubMed - DOI
    1. Gong, J. S., Lu, Z. M., Li, H., Shi, J. S., Zhou, Z. M., & Xu, Z. H. (2012). Nitrilases in nitrile biocatalysis: Recent progress and forthcoming research. Microbial Cell Factories, 11, 142–148. - PubMed - PMC - DOI

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