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. 2023 Nov;36(6):2195-2203.
doi: 10.1007/s13577-023-00944-0. Epub 2023 Jul 15.

Establishment and characterization of a novel dedifferentiated chondrosarcoma cell line, SMU-DDCS, harboring an IDH1 mutation

Makoto Emori  1 , Naoya Nakahashi  2   3 , Akira Takasawa  3 , Kenji Murata  3 , Yasutaka Murahashi  2 , Junya Shimizu  2 , Tomohide Tsukahara  3 , Shintaro Sugita  4 , Kohichi Takada  5 , Tadashi Hasegawa  4 , Makoto Osanai  3 , Kosuke Iba  6
Affiliations

Establishment and characterization of a novel dedifferentiated chondrosarcoma cell line, SMU-DDCS, harboring an IDH1 mutation

Makoto Emori et al. Hum Cell. 2023 Nov.

Abstract

Dedifferentiated chondrosarcoma (DDCS) is a high-grade subtype with a bi-morphic histological appearance of a conventional chondrosarcoma component and it can abruptly transition to a high-grade non-cartilaginous sarcoma. To better understand the biological features of DDCSs and to help develop new therapies, a novel DDCS cell line, SMU-DDCS, was established. Tissue from an open biopsy of a tumor resected from a 75-year-old patient was subjected to primary culture. The cell line was established and authenticated by assessing DNA microsatellite short tandem repeats. The cells maintained in monolayer cultures exhibited constant growth, spheroid formation, and high invasive capacity. Out of the four mice inoculated with SMU-DDCS cells, tumors developed in three mice after 2 weeks. R132C mutation was found in the IDH1 but not the IDH2 genomic DNA sequence of SMU-DDCS cells. SMU-DDCS cells exhibited low chemosensitivity to doxorubicin, methotrexate, and cisplatin. This SMU-DDCS cell line harboring an IDH1 mutation will be a useful tool for investigating DDCS development and for evaluating novel therapeutic agents against it.

Keywords: Cell line; Dedifferentiated chondrosarcoma; Isocitrate dehydrogenase.

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References

    1. Thorkildsen J, Taksdal I, Bjerkehagen B, Haugland HK, Børge Johannesen T, Viset T, et al. Chondrosarcoma in Norway 1990–2013; an epidemiological and prognostic observational study of a complete national cohort. Acta Oncol. 2019;58(3):273–82. https://doi.org/10.1080/0284186x.2018.1554260 . - DOI - PubMed
    1. . WHO Classification of Tumours of Soft Tissue and Bone. Lyon: IARC Press; 2020. p. 370–90.
    1. Grimer RJ, Gosheger G, Taminiau A, Biau D, Matejovsky Z, Kollender Y, et al. Dedifferentiated chondrosarcoma: prognostic factors and outcome from a European group. Eur J Cancer. 2007;43(14):2060–5. https://doi.org/10.1016/j.ejca.2007.06.016 . - DOI - PubMed
    1. Miao R, Choy E, Raskin KA, Schwab JH, Nielsen GP, Deshpande V, et al. Prognostic factors in dedifferentiated chondrosarcoma: a retrospective analysis of a large series treated at a single institution. Sarcoma. 2019;2019:9069272. https://doi.org/10.1155/2019/9069272 . - DOI - PubMed - PMC
    1. Kudo N, Ogose A, Hotta T, Kawashima H, Gu W, Umezu H, et al. Establishment of novel human dedifferentiated chondrosarcoma cell line with osteoblastic differentiation. Virchows Arch. 2007;451(3):691–9. https://doi.org/10.1007/s00428-007-0426-3 . - DOI - PubMed

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