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. 2023:2677:99-112.
doi: 10.1007/978-1-0716-3259-8_5.

Targeting Endogenous Loci That Function in Drosophila Germline Stem Cells

Marianne Mercer  1 , Varsha Bhargava  1 , Courtney D Goldstein  1 , Michael Buszczak  2
Affiliations

Targeting Endogenous Loci That Function in Drosophila Germline Stem Cells

Marianne Mercer et al. Methods Mol Biol. 2023.

Abstract

CRISPR-Cas9 genome editing technology can be used to manipulate the genome of Drosophila melanogaster. The ability to delete genes, make specific mutations, add tags, or make other genetic manipulations is useful for studying germline stem cell biology. In this chapter, we will describe a method to use CRISPR-Cas9 genome editing technology to make knock-out and knock-in flies. We will cover everything from guideRNA (gRNA) and donor plasmid design and cloning to screening for positive edits.

Keywords: CRISPR-Cas9; Drosophila ovary; Genome editing; Germline stem cells.

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