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Review
. 2023 Jul 4:14:1193213.
doi: 10.3389/fphar.2023.1193213. eCollection 2023.

Overview of research progress and application of experimental models of colorectal cancer

Li Liu  1   2   3 , Qiuying Yan  2   3 , Zihan Chen  1 , Xiaoman Wei  2 , Lin Li  1 , Dongxin Tang  4 , Jiani Tan  2   3 , Changliang Xu  2   3 , Chengtao Yu  2   3 , Yueyang Lai  2   3 , Minmin Fan  2   3 , Lihuiping Tao  2   3 , Weixing Shen  2   3 , Liu Li  2   3 , Mianhua Wu  2 , Haibo Cheng  2   3 , Dongdong Sun  1   2   3
Affiliations
Review

Overview of research progress and application of experimental models of colorectal cancer

Li Liu et al. Front Pharmacol. .

Abstract

Colorectal cancer (CRC) is the third most common malignancy in terms of global tumor incidence, and the rates of morbidity and mortality due to CRC are rising. Experimental models of CRC play a vital role in CRC research. Clinical studies aimed at investigating the evolution and mechanism underlying the formation of CRC are based on cellular and animal models with broad applications. The present review classifies the different experimental models used in CRC research, and describes the characteristics and limitations of these models by comparing the research models with the clinical symptoms. The review also discusses the future prospects of developing new experimental models of CRC.

Keywords: animal models; cellular models; colorectal cancer; drug development; preclinical studies.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
History of development of in vitro models of CRC.
FIGURE 2
FIGURE 2
For the formation process of spherical cancer models (A) MCTS: Cell suspensions cultured under non-adherent conditions were aggregated and compacted to obtain MCTS; (B) Tumorospheres: Stem cells cultured under low-adherent conditions formed Tumorospheres by clonal proliferation (C) TDTS: Partial dissociation of tumor tissue and compaction/remodeling produced TDTS; (D) OMS: Cut tumor tissue aggregates formed OMS during culture under non-adherent conditions.
FIGURE 3
FIGURE 3
Intestine organoid cultures.
FIGURE 4
FIGURE 4
Application of CRC cellular models.
FIGURE 5
FIGURE 5
Chemically induced CRC animal models.
See this image and copyright information in PMC

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