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. 2023 Jun;175(2):239-244.
doi: 10.1007/s10517-023-05842-x. Epub 2023 Jul 21.

Identification of Chimeric NTRK3 Genes in Papillary Thyroid Cancer Cells by Analyzing the Imbalance of the Expression of 5' and 3' mRNA Fragments

I P Oskorbin  1 , A A Ivanov  2 , M A Smertina  1 , I A Demidova  3 , U A Boyarskikh  1 , A A Kechin  1 , S Yu Bakharev  2 , O V Samuilenkova  2 , I V Vihlyanov  2 , N E Kushlinskii  4 , M L Filipenko  5
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Identification of Chimeric NTRK3 Genes in Papillary Thyroid Cancer Cells by Analyzing the Imbalance of the Expression of 5' and 3' mRNA Fragments

I P Oskorbin et al. Bull Exp Biol Med. 2023 Jun.

Abstract

The standard for detecting chimeric genes of neurotrophic receptor tyrosine kinases (NTRK) is next generation sequencing (NGS). However, this analysis is expensive and takes several days. As a rapid screening method for the detection of NTRK3-dependent papillary thyroid cancer, an analysis of the expression imbalance between 5' and 3' NTRK3 mRNA fragments was used (5'/3' RT-PCR). The reference method for detection of NTRK3 rearrangements was fluorescent in situ hybridization (FISH), and the most frequent rearrangements in papillary thyroid cancer were tested using reverse transcription PCR (RT-PCR). Using 5'/3' RT-PCR, 18 samples of papillary thyroid cancer carrying chimeric transcripts of NTRK3 mRNA were detected. The sensitivity of the developed technique was 88.9% and specificity was 99.3%. Thus, a fast and cost-effective method of screening samples of papillary thyroid cancer in paraffin blocks is proposed with acceptable sensitivity and specificity.

Keywords: NTRK3; RT-PCR; TRK; chimeric mRNA; papillary thyroid cancer.

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