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. 2023 Sep;50(9):7479-7487.
doi: 10.1007/s11033-023-08626-8. Epub 2023 Jul 22.

Gene expression study of host-human T-cell leukaemia virus type 1 (HTLV-1) interactions: adult T-cell leukaemia/lymphoma (ATLL)

Masooma Rahimzada  1 , Mehri Nahavandi  2   3 , Mona Saffari  1 , Azam Shafaei  4 , Arman Mosavat  4 , Sanaz Ahmadi Gezeldasht  4 , Nazila Ariaee  1 , Narges Valizadeh  1 , Hossein Rahimi  5 , Seyed Abdolrahim Rezaee  6 , Mohammad Derakhshan  7   8
Affiliations

Gene expression study of host-human T-cell leukaemia virus type 1 (HTLV-1) interactions: adult T-cell leukaemia/lymphoma (ATLL)

Masooma Rahimzada et al. Mol Biol Rep. 2023 Sep.

Abstract

Background: In HTLV-1-associated malignant disease, adult T-cell leukaemia/lymphoma (ATLL), the interaction of virus and host was evaluated at the chemokines gene expression level. Also, IL-1β and Caspase-1 expressions were evaluated to investigate the importance of pyroptosis in disease development and progression.

Methods and results: The expression of host CCR6 and CXCR-3 and the HTLV-1 proviral load (PVL), Tax, and HBZ were assessed in 17 HTLV-1 asymptomatic carriers (ACs) and 12 ATLL patients using the reverse transcription-quantitative polymerase chain reaction (RT-qPCR), TaqMan method. Moreover, RT-qPCR, SYBR Green assay were performed to measure Caspase-1 and IL-1β expression. HTLV-1-Tax did not express in 91.5% of the ATLLs, while HBZ was expressed in all ATLLs. The expression of CXCR3 dramatically decreased in ATLLs compared to ACs (p = 0.001). The expression of CCR6 was lower in ATLLs than ACs (p = 0.04). The mean of PVL in ATLL patients was statistically higher than ACs (p = 0.001). Furthermore, the expression of the IL-1β between ATLLs and ACs was not statistically significant (p = 0.4). In contrast, there was a meaningful difference between Caspase-1 in ATLLs and ACs (p = 0.02).

Conclusions: The present study indicated that in the first stage of ATLL malignancy toward acute lymphomatous, CXCR3 and its progression phase may target the pyroptosis process. Mainly, HBZ expression could be a novel therapeutic target.

Keywords: ATLL; CXCR3; HBZ; HTLV-1; Pyroptosis; Tax.

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