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. 2023 Jul 22;205(8):295.
doi: 10.1007/s00203-023-03630-9.

Effect of honey bee forage plants in Tunisia on diversity and antibacterial potential of lactic acid bacteria and bifidobacteria from Apis mellifera intermissa and its products

Houda Ben-Miled  1 , Nabil Semmar  2 , Miguel Sautié Castellanos  3 , Kamel Ben-Mahrez  1 , Marie-Odile Benoit-Biancamano  4 , Samia Réjiba  5   6   7
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Effect of honey bee forage plants in Tunisia on diversity and antibacterial potential of lactic acid bacteria and bifidobacteria from Apis mellifera intermissa and its products

Houda Ben-Miled et al. Arch Microbiol. .

Abstract

Lactic acid bacteria and bifidobacteria (LAB and Bifido), isolated from the gastrointestinal tract of Apis mellifera intermissa (BGIT), honey (H), propolis (P) and bee bread (BB) of hives set in different vegetations (wildflowers, caraway, orange blossom, Marrubium vulgare, Eucalyptus and Erica cinerea), were subjected to analysis of their antibacterial potential. Isolates able to inhibit Staphylococcus aureus were selected and identified with MALDI-TOF MS leading to 154 strains representing 12 LAB and Bifido species. Lactiplantibacillus plantarum, Pediococcus pentosaceus and Enterococcus faecalis were predominantly found in all matrices. BGIT showed the highest LAB and Bifido diversity with exclusive occurrences of five species (including Bifidobacterium asteroides and Limosilactobacillus fermentum). Honey was the second origin harboring an important variety of LAB species of which Apilactobacillus kunkeei and Enterococcus mundtii were characteristic of both H and BGIT. Principal components analysis revealed associations between antibacterial activities of LAB and Bifido, matrices and honey bee forage plants. Inhibition trends of S. aureus and Citrobacter freundii were highlighted with: L. plantarum from BGIT, P, H of bees feeding on E. cinerea; Pediococcus pentosaceus from BGIT, P, BB associated with E. cinerea; and Bifidobacterium asteroides from BGIT/orange blossom system. However, Enterococcus faecium associated with BGIT/Eucalyptus system antagonized Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter baumannii. Our findings highlighted noteworthy effects of bee forage plants on the antibacterial activity of LAB and Bifido. Our approach could be useful to identify multiple conditions promoting antibacterial potency of LAB and Bifido under the combined effects of feeding plants and living matrices.

Keywords: Antibacterial potential; Bee products; Bifidobacteria; Forage plants; Honey bee; Lactic acid bacteria.

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References

    1. Abriouel H, Lerma LL, Casado Muñoz M, Montoro BP, Kabisch J, Pichner R, Cho GS, Neve H, Fusco V, Franz CM, Gálvez A, Benomar N (2015) The controversial nature of the Weissella genus: technological and functional aspects versus whole genome analysis-based pathogenic potential for their application in food and health. Front Microbiol 6:1197. https://doi.org/10.3389/fmicb.2015.01197 - DOI - PubMed - PMC
    1. Ağagündüz D, Yılmaz B, Şahin TÖ, Güneşliol BE, Ayten Ş, Russo P, Spano G, Rocha JM, Bartkiene E, Özogul F (2021) Dairy lactic acid bacteria and their potential function in dietetics: the food-gut-health axis. Foods 10(12):3099. https://doi.org/10.3390/foods10123099 - DOI - PubMed - PMC
    1. Anderson KE, Ricigliano VA, Copeland DC, Mott BM, Maes P (2023) Social interaction is unnecessary for hindgut microbiome transmission in honey bees: the effect of diet and social exposure on tissue-specific microbiome assembly. Microb Ecol 85(4):1498–1513. https://doi.org/10.1007/s00248-022-02025-5 - DOI - PubMed
    1. Asama T, Arima TH, Gomi T, Keishi T, Tani H, Kimura Y, Tatefuji T, Hashimoto K (2015) Lactobacillus kunkeei YB38 from honeybee products enhances IgA production in healthy adults. J Appl Microbiol 119:818–826. https://doi.org/10.1111/jam.12889 - DOI - PubMed
    1. Bottacini F, Ventura M, van Sinderen D, O’Connell Motherway M (2014) Diversity, ecology and intestinal function of bifidobacteria. Microb Cell Fact 13(Suppl 1):S4. https://doi.org/10.1186/1475-2859-13-S1-S4 - DOI - PubMed - PMC

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