Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Aug 11;14(15):6145-58.
doi: 10.1093/nar/14.15.6145.

Transcriptional inactivity of Alu repeats in HeLa cells

K E Paulson, C W Schmid
Free PMC article

Transcriptional inactivity of Alu repeats in HeLa cells

K E Paulson et al. Nucleic Acids Res. .
Free PMC article

Abstract

The in vivo transcription of human Alu family members has been investigated by a sensitive primer extension method. The selected primers represent various regions of the Alu family consensus sequence, thus assaying the transcriptional activity of the entire family rather than the activity of an individual member sequence. Using this method, a very small number of RNA molecules per HeLa cell is found to have a distribution of 5' ends centered on the in vitro Alu transcription start site. The distribution of these 5' ends suggests that they are more likely the result of hnRNA degradation rather than transcription start sites. Therefore, despite their great numerical abundance, Alu family members are transcriptionally silent in HeLa cells.

PubMed Disclaimer

References

    1. J Mol Biol. 1968 Dec;38(3):289-304 - PubMed
    1. Mol Cell Biol. 1986 Feb;6(2):425-40 - PubMed
    1. Biochemistry. 1978 Mar 21;17(6):1073-80 - PubMed
    1. Biochemistry. 1978 Jul 11;17(14):2776-83 - PubMed
    1. Cell. 1978 Sep;15(1):205-14 - PubMed

Publication types