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. 1986 Aug 11;14(15):6227-45.
doi: 10.1093/nar/14.15.6227.

Efficient methods for attaching non-radioactive labels to the 5' ends of synthetic oligodeoxyribonucleotides

S Agrawal, C Christodoulou, M J Gait
Free PMC article

Efficient methods for attaching non-radioactive labels to the 5' ends of synthetic oligodeoxyribonucleotides

S Agrawal et al. Nucleic Acids Res. .
Free PMC article

Abstract

The syntheses are described of two types of linker molecule useful for the specific attachment of non-radioactive labels such as biotin and fluorophores to the 5' terminus of synthetic oligodeoxyribonucleotides. The linkers are designed such that they can be coupled to the oligonucleotide as a final step in solid-phase synthesis using commercial DNA synthesis machines. Increased sensitivity of biotin detection was possible using an anti-biotin hybridoma/peroxidase detection system.

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