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. 1986 Aug 26;14(16):6711-9.
doi: 10.1093/nar/14.16.6711.

Caenorhabditis elegans DNA does not contain 5-methylcytosine at any time during development or aging

V J Simpson, T E Johnson, R F Hammen
Free PMC article

Caenorhabditis elegans DNA does not contain 5-methylcytosine at any time during development or aging

V J Simpson et al. Nucleic Acids Res. .
Free PMC article

Abstract

DNA, isolated from age-synchronous senescent populations of Caenorhabditis elegans has been quantitatively and qualitatively analyzed for the presence of 5-methylcytosine. High performance liquid chromatography on two wild-type and several mutant strains of C. elegans failed to detect any 5-methylcytosine. The restriction endonuclease isoschizomers, HpaII and MspI, were used to digest genomic DNA after CsCl purification and failed to detect any 5' cytosine methylation at any age. We conclude that C. elegans does not contain detectable (0.01 mole percent) levels of 5-methylcytosine.

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