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. 2023 Jul 27;13(1):12212.
doi: 10.1038/s41598-023-39454-2.

Draft genome sequence and probiotic functional property analysis of Lactobacillus gasseri LM1065 for food industry applications

Won-Young Bae  1 , Young Jin Lee  2 , Woo-Hyun Jung  2 , So Lim Shin  2 , Tae-Rahk Kim  2 , Minn Sohn  2
Affiliations

Draft genome sequence and probiotic functional property analysis of Lactobacillus gasseri LM1065 for food industry applications

Won-Young Bae et al. Sci Rep. .

Abstract

Probiotics are defined as live organisms in the host that contribute to health benefits. Lactobacillus gasseri LM1065, isolated from human breast milk, was investigated for its probiotic properties based on its genome. Draft genome map and de novo assembly were performed using the PacBio RS II system and hierarchical genome assembly process (HGAP). Probiotic properties were determined by the resistance to gastric conditions, adherence ability, enzyme production, safety assessment and mobile genetic elements. The fungistatic effect and inhibition of hyphae transition were studied using the cell-free supernatant (CFS). L. gasseri LM1065 showed high gastric pepsin tolerance and mild tolerance to bile salts. Auto-aggregation and hydrophobicity were measured to be 61.21% and 61.55%, respectively. The adherence to the human intestinal epithelial cells was measured to be 2.02%. Antibiotic-resistance genes and putative virulence genes were not predicted in the genomic analysis, and antibiotic susceptibility was satisfied by the criteria of the European Food Safety Authority. CFS showed a fungistatic effect and suppressed the tricarboxylic acid cycle in Candida albicans (29.02%). CFS also inhibited the transition to true hyphae and damaged the blastoconidia. This study demonstrates the essential properties of this novel probiotic, L. gasseri LM1065, and potential to inhibit vaginal C. albicans infection.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Circular gene map, phylogenetic relationship, and Gene Ontology of Lactobacillus gasseri LM1065. (A) Circular gene map of three contigs. Each circle from outside to inside indicates protein-coding sequences (CDS) on forward strand, CDS on reverse strand, tRNA, rRNA, GC content, and GC skew. (B) Phylogenetic tree. (C) Average nucleotide identity (ANI) value. (D) Gene Ontology. (E) Bacteriocin gene cluster.
Figure 2
Figure 2
Multiple genome alignment and genomic comparison of Lactobacillus gasseri.
Figure 3
Figure 3
Tricarboxylic acid cycle activity in Candida albicans ATCC 11006 treated by cell free supernatant. Significant differences within test groups were analyzed Tukey’s range tests (P < 0.001).
Figure 4
Figure 4
Microscopic features of Candida albicans ATCC 11006 treated by cell free supernatant (observed at the magnification of 400 ×).
See this image and copyright information in PMC

References

    1. Garcia-Gutierrez E, et al. Production of multiple bacteriocins, including the novel bacteriocin gassericin M, by Lactobacillus gasseri LM19, a strain isolated from human milk. Appl. Microbiol. Biotechnol. 2020;104:3869–3884. doi: 10.1007/s00253-020-10493-3. - DOI - PMC - PubMed
    1. Gunyakti A, Asan-Ozusaglam M. Lactobacillus gasseri from human milk with probiotic potential and some technological properties. LWT Food Sci. Technol. 2019;109:261–269. doi: 10.1016/j.lwt.2019.04.043. - DOI
    1. Zhou X, et al. Comparative analysis of Lactobacillus gasseri from Chinese subjects reveals a new species-level taxa. BMC Genomics. 2020;21:119. doi: 10.1186/s12864-020-6527-y. - DOI - PMC - PubMed
    1. Zhou QY, et al. Lactobacillus gasseri LGZ 1029 in yogurt: Rheological behaviour and volatile compound composition. Int. J. Food Sci. Technol. 2021;56:2992–3003. doi: 10.1111/ijfs.14942. - DOI
    1. Atassi F, Pho Viet Ahn DL, Lievin-Le Moal V. Diverse expression of antimicrobial activities against bacterial vaginosis and urinary tract infection pathogens by cervicovaginal microbiota strains of Lactobacillus gasseri and Lactobacillus crispatus. Front. Microbiol. 2019;10:2900. doi: 10.3389/fmicb.2019.02900. - DOI - PMC - PubMed

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