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. 2024 Jan 1;32(1):146-153.
doi: 10.4062/biomolther.2023.116. Epub 2023 Jul 28.

Association between LEPR Genotype and Gut Microbiome in Healthy Non-Obese Korean Adults

Yoon Jung Cha  1   2 , In Ae Chang  1 , Eun-Heui Jin  3 , Ji Hye Song  1   2 , Jang Hee Hong  1   4 , Jin-Gyu Jung  5 , Jung Sunwoo  1
Affiliations

Association between LEPR Genotype and Gut Microbiome in Healthy Non-Obese Korean Adults

Yoon Jung Cha et al. Biomol Ther (Seoul). .

Abstract

The LEPR (leptin receptor) genotype is associated with obesity. Gut microbiome composition differs between obese and non-obese adults. However, the impact of LEPR genotype on gut microbiome composition in humans has not yet been studied. In this study, the association between LEPR single nucleotide polymorphism (rs1173100, rs1137101, and rs790419) and the gut microbiome composition in 65 non-obese Korean adults was investigated. Leptin, triglyceride, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol levels were also measured in all participants. Mean ± SD (standard deviation) of age, body mass index, and leptin hormone levels of participants was 35.2 ± 8.1 years, 21.4 ± 1.8 kg/m2, and 7989.1 ± 6687.4 pg/mL, respectively. Gut microbiome analysis was performed at the phylum level by 16S rRNA sequencing. Among the 11 phyla detected, only one showed significantly different relative abundances between LEPR genotypes. The relative abundance of Candidatus Saccharibacteria was higher in the G/A genotype group than in the G/G genotype group for the rs1137101 single nucleotide polymorphism (p=0.0322). Participant characteristics, including body mass index, leptin levels, and other lipid levels, were similar between the rs1137101 G/G and G/A genotypes. In addition, the relative abundances of Fusobacteria and Tenericutes showed significant positive relationship with plasma leptin concentrations (p=0.0036 and p=0.0000, respectively). In conclusion, LEPR genotype and gut microbiome may be associated even in normal-weight Korean adults. However, further studies with a greater number of obese adults are needed to confirm whether LEPR genotype is related to gut microbiome composition.

Keywords: Gastrointestinal microbiome; Industrial microbiology; Leptin; Obesity; Pharmacogenetics; Receptors; leptin.

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Conflict of interest statement

CONFLICT OF INTEREST

All authors declare no conflict of interest.

Figures

Fig. 1
Fig. 1
The average phylum distribution of the gut microbiome in non-obese Korean adults (N=65).
Fig. 2
Fig. 2
The average species distribution of the gut microbiome in non-obese Korean adults, based on species with a mean percentage above 1% (N=65).
Fig. 3
Fig. 3
Comparison of relative abundance of Candidatus Saccharibacteria according to LEPR rs1137101 genotype (Mann–Whitney test).
Fig. 4
Fig. 4
Association between leptin concentration and relative abundance of Fusobacteria (A) and Ternericutes (B) in non-obese Korean adults. Each black circle in the graph represents an individual participant (N=65).
Fig. 5
Fig. 5
Relative abundance association between each phylum in non-obese Korean adults (N=65).
Fig. 6
Fig. 6
Association between low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and triglyceride levels in non-obese Korean adults (N=65).
See this image and copyright information in PMC

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