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. 2023 Jun 30;13(7):640.
doi: 10.3390/membranes13070640.

Evaluation of the Ability of PAMPA Membranes to Emulate Biological Processes through the Abraham Solvation Parameter Model

Sara Soriano-Meseguer  1 , Elisabet Fuguet  1   2   3 , Adriana Port  4 , Martí Rosés  1   2
Affiliations

Evaluation of the Ability of PAMPA Membranes to Emulate Biological Processes through the Abraham Solvation Parameter Model

Sara Soriano-Meseguer et al. Membranes (Basel). .

Abstract

Two parallel artificial membrane permeability assay (PAMPA) systems intended for emulating skin permeability have been characterized through the solvation parameter model of Abraham using multilinear regression analysis. The coefficients of the obtained equations have been compared to the ones already established for other PAMPA membranes using statistical tools. The results indicate that both skin membranes are similar to each other in their physicochemical properties. However, they are different from other PAMPA membranes (e.g., intestinal absorption and blood-brain PAMPAs), mainly in terms of hydrophobicity and hydrogen bonding properties. Next, all PAMPA membranes have been compared to relevant biological processes also characterized through the solvation parameter model. The results highlight that skin-PAMPA membranes are a very good choice to emulate skin permeability.

Keywords: PAMPA; blood-brain partition; blood-brain permeation; human intestinal absorption; prediction; skin permeation; solvation parameter model.

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Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

Figures

Figure 1
Figure 1
Radial plot according to the coefficient values for the different PAMPA systems: PAMPA-Certramide (1, —); PAMPA-IPM (2, —); PAMPA-BBB (3, —); PAMPA-HDM (4, —); PAMPA-DOPC (5, —); PAMPA-DS (6, —); PAMPA-P0 (7, —); PAMPA-COS (8, —); PAMPA-P16 (9, —).
Figure 2
Figure 2
Dendrogram between the different PAMPA systems.
Figure 3
Figure 3
PCA analysis of the different PAMPA systems. Clusters with D′ < 1.0 (– – –) and clusters with D′ between 1 and 2 (– · –).
See this image and copyright information in PMC

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