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. 2023 Jul 10;13(14):2254.
doi: 10.3390/ani13142254.

De Novo Assembly, Characterization and Comparative Transcriptome Analysis of the Gonads of Jade Perch (Scortum barcoo)

Shiyan Liu  1 , Yingying Lian  1 , Yikun Song  1 , Qinghua Chen  2 , Jianrong Huang  1
Affiliations

De Novo Assembly, Characterization and Comparative Transcriptome Analysis of the Gonads of Jade Perch (Scortum barcoo)

Shiyan Liu et al. Animals (Basel). .

Abstract

Due to the high meat yield and rich nutritional content, jade perch (Scortum barcoo) has become an important commercial aquaculture species in China. Jade perch has a slow growth rate, taking 3-4 years to reach sexual maturity, and has almost no difference in body size between males and females. However, the study of its gonad development and reproduction regulation is still blank, which limited the yield increase. Herein, the gonad transcriptomes of juvenile males and females of S. barcoo were identified for the first time. A total of 107,060 unigenes were successfully annotated. By comparing male and female gonad transcriptomes, a total of 23,849 differentially expressed genes (DEGs) were identified, of which 9517 were downregulated, and 14,332 were upregulated in the testis. In addition, a large number of DEGs involved in sex differentiation, gonadal development and differentiation and gametogenesis were identified, and the differential expression patterns of some genes were further verified using real-time fluorescence quantitative PCR. The results of this study will provide a valuable resource for further studies on sex determination and gonadal development of S. barcoo.

Keywords: Scortum barcoo; gonad differentiation and development; jade perch; sex-related genes; transcriptome.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Length distribution of assembled unigenes of the jade perch gonadal transcriptome. X-axis: size of unigene; Y-axis: number of unigenes.
Figure 2
Figure 2
The species distribution of the results of Nr annotation. X-axis: the top species which match the annotated sequences distribution. Y-axis: the number of annotated sequences matching each species.
Figure 3
Figure 3
Function annotation of unigenes based on GO (A), KEGG (B) and KOG (C) databases.
Figure 4
Figure 4
Volcano plot of DEGs in testes versus ovaries. Upregulated and downregulated genes were respectively represented by red and green points in males and vice versa.
Figure 5
Figure 5
KEGG enrichment analysis of the top 20 pathways. X-axis: the ratio of the number of differential genes annotated in the KEGG pathways to the whole number of differential genes; Y-axis: the name of the enriched KEGG pathways. The sizes of the dots represent the count of the differential genes, and the color of the dots represents the significant enrichment from red to blue.
Figure 6
Figure 6
Expression patterns verification of 10 testes-biased and eight ovaries-biased genes (A) by qRT-PCR. Correlation analysis of the RNA-Seq data and qRT-PCR data (B).
Figure 7
Figure 7
Testis (A) and ovary (B) histology of jade perch. SG: primary spermatogonia; PG: primary growth oocyte. Scale bars: 10 μm.
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