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. 2023 Jul 20;12(7):1211.
doi: 10.3390/antibiotics12071211.

Isolation, Characterization of Pyraclostrobin Derived from Soil Actinomycete Streptomyces sp. HSN-01 and Its Antimicrobial and Anticancer Activity

Halaswamy Hire Math  1 , Sreenivasa Nayaka  1 , Muthuraj Rudrappa  1 , Raju Suresh Kumar  2 , Abdulrahman I Almansour  2 , Karthikeyan Perumal  3 , Girish Babu Kantli  4
Affiliations

Isolation, Characterization of Pyraclostrobin Derived from Soil Actinomycete Streptomyces sp. HSN-01 and Its Antimicrobial and Anticancer Activity

Halaswamy Hire Math et al. Antibiotics (Basel). .

Abstract

The present study demonstrated the isolation, characterization, and antimicrobial and anticancer activity of active metabolite produced from mining-soil-derived actinomycetes. Among the 21 actinomycete isolates, the isolate HSN-01 exhibited significant antimicrobial activity in primary screening and was identified as Streptomyces sp. through 16S rRNA gene sequencing. The active metabolite was separated, purified, and confirmed through UV-Vis spectroscopy, FTIR, HR-ESI-MS, and NMR analysis and identified as pyraclostrobin. Further, the active metabolite pyraclostrobin was tested for antimicrobial and anticancer activity against the hepatocellular carcinoma (HepG2) cell line. The metabolite exhibited maximum antimicrobial potential with 17.0, 13.33, 17.66, 15.66, 14.66, and 14.0 mm of inhibition against B. cereus, S. aureus, E. coli, P. aeruginosa, S. flexneri, and C. glabrata. The active metabolite exhibited dose-dependent anticancer potential against the hepatocellular carcinoma (HepG2) cell line with the IC50 56.76 µg/mL. This study suggests that Streptomyces sp. HSN-01 is an excellent source of active secondary metabolites with various biological activities.

Keywords: NMR; Streptomyces sp.; anticancer activity; antimicrobial activity; pyraclostrobin.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Dendrogram indicating the phylogenetic relation of the isolate HSN-01 with closely related Streptomyces species.
Figure 2
Figure 2
Colony morphology; (A) aerial mycelium, (B) substrate mycelium, and (C) SEM image showing spore chains and spore surface of Streptomyces sp. HSN-01.
Figure 3
Figure 3
UV–Vis spectrum of pyraclostrobin.
Figure 4
Figure 4
FTIR spectrum of pyraclostrobin isolated from Streptomyces sp. HSN-01.
Figure 5
Figure 5
HR-ESI-MS spectrum of pyraclostrobin showing a molecular peak at m/z 388.1686 [M+H]+.
Figure 6
Figure 6
Elusion profiles; (A) standard pyraclostrobin (tR = 5.12 min) and (B) pyraclostrobin from Streptomyces sp. HSN-01 (tR = 5.11 min).
Figure 7
Figure 7
1H NMR spectrum of pyraclostrobin.
Figure 8
Figure 8
13C NMR spectrum of pyraclostrobin.
Figure 9
Figure 9
Chemical structure of the compound pyraclostrobin.
Figure 10
Figure 10
Antimicrobial activity of pyraclostrobin by agar well diffusion method; (A) B. cereus, (B) S. aureus, (C) E. coli, (D) P. aeruginosa, (E) S. flexneri, (F) C. glabrata. (G) Bar graph showing zones of inhibition of pathogens at different concentrations of pyraclostrobin.
Figure 11
Figure 11
In vitro anticancer activity of purified compound at different concentrations against HepG2 cell line; (A) untreated, (B) standard control (camptothecin), (C) 12.5 µg/mL, (D) 25 µg/mL, (E) 50 µg/mL, (F) 100 µg/mL, (G) 200 µg/mL. (H) Comparative % cell viability of HepG2 cells treated with different concentrations of purified compound (scale bar: 200 µm).
See this image and copyright information in PMC

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