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. 2023 Jul 20;13(7):1161.
doi: 10.3390/jpm13071161.

Evaluation of microRNA Expression Features in Patients with Various Types of Arterial Damage: Thoracic Aortic Aneurysm and Coronary Atherosclerosis

Ange Veroniqe Ngo Bilong Ekedi  1 , Andrey N Rozhkov  2 , Dmitry Yu Shchekochikhin  1 , Nina A Novikova  1 , Philippe Yu Kopylov  1   2 , Afina A Bestavashvili  2 , Tatiana V Ivanova  1 , Andrey V Zhelankin  3 , Eduard V Generozov  3 , Dmitry N Konanov  3 , Anna S Akselrod  1
Affiliations

Evaluation of microRNA Expression Features in Patients with Various Types of Arterial Damage: Thoracic Aortic Aneurysm and Coronary Atherosclerosis

Ange Veroniqe Ngo Bilong Ekedi et al. J Pers Med. .

Abstract

Circulating serum miRNA are increasingly used as biomarkers and potential treatment targets in several clinical scenarios, including cardiovascular diseases. However, the current data on circulating miRNA in thoracic aorta aneurism (TAA) patients are inconclusive. The aim of the present study is to compare the levels of several circulating miRNA in patients with degenerative TAA, coronary artery disease (CAD), and controls for special profile identification. We have identified several candidates for the role of new biomarkers: miR-143-3p, miR-181-5p, miR-126-3p, miR-126-5p, miR-145-5p, miR-150-5p, and miR-195-5p.

Materials and methods: Serum samples of 100 patients were analyzed, including 388 TAA patients scheduled for elective surgery, 67 patients with stable CAD and 17 controls, were used for miRNA isolation and identification.

Results: More specific for TAA with very high predictive ability in ROC analysis was an increase in the levels of miR-21-5p, miR-29b-5p, miR-126-5p/-3p, miR-181b-5p, and miR-92a-3p, with the latter microRNA being investigated as a novel potential marker of TAA for the first time.

Conclusion: TAA and CAD patients demonstrated a significant increase in the levels of circulating miR-126-5p/-3p, miR-181b-5p, and miR-29b-3p. More specific for TAA with very high predictive ability in ROC analysis was an increase in the levels of miR-21-5p, -29b-5p, -126-5p/-3p, 181b-5p, and -92a-3p, with the latter microRNA being investigated as a potential marker of TAA for the first time.

Keywords: HDL; atherosclerosis; miR-126; miR-181b-5p; miR-21-5p; miR-29b-3p; miR-92a-3p; microRNA; thoracic aorta aneurism.

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Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

Figures

Figure 1
Figure 1
Block diagram of the study.
Figure 2
Figure 2
Results of comparison of miRNA relative plasma levels in patients with damaged arteries.
Figure 3
Figure 3
Results of sensitivity and specificity analysis of miR-126-5p, miR-126-3p, miR-181b-5p, and -29b-3p relative plasma levels increase as a marker of arteries damage. ROC curves.
Figure 4
Figure 4
Results of sensitivity and specificity analysis with AUC > 0.8 of the selected miRNA relative plasma levels increase as a marker of TAA. ROC curves.
Figure 5
Figure 5
Results of comparison of miRNA relative plasma levels in patients with and without CAD.
Figure 6
Figure 6
Results of sensitivity and specificity analysis of the selected miRNA relative plasma levels increase as a marker of CAD. ROC curves.
Figure 7
Figure 7
Spearman rank correlation matrix of selected clinical parameters with relative plasma levels of miRNA adjusted for multiple comparisons.
Figure 8
Figure 8
Pearson rank correlation matrix of selected clinical parameters with relative plasma levels of miRNA adjusted for multiple comparisons.
Figure 9
Figure 9
Association of HDL levels with the presence of TAA.
See this image and copyright information in PMC

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