Isolation of Trypanosoma cruzi from the blood of infected mice by column chromatography
- PMID: 375218
- DOI: 10.1080/00327487908061675
Isolation of Trypanosoma cruzi from the blood of infected mice by column chromatography
Abstract
Trypomastogotes of three strains of Trypanosoma cruzi were isolated from the blood of infected mice employing lymphoprep for separation of the red blood cells and a column of DEAE cellulose for removal of white cells and platelets. An average recovery of 45 to 58 percent of actively motile, infective organisms, free of contaminating blood cells was obtained. Protein and carbohydrate assays of the separated organisms revealed significant differences between the Tulahuen, a reticulotropic strain, and the House 510 and House 11, two myotropic strains of this parasitic species. The present procedure should provide sufficient parasites for physiological and biochemical studies; it has also served to indicate particular strain characteristics which may aid in a taxonomic classification of these organisms.
Similar articles
-
Trypanosoma cruzi: selective isolation of pure trypomastigotes from cultured muscle cells.J Parasitol. 1981 Aug;67(4):517-21. J Parasitol. 1981. PMID: 7021789
-
Effect of purification by DEAE-cellulose column on infectivity of Trypanosoma cruzi blood forms.J Parasitol. 1979 Feb;65(1):188-9. J Parasitol. 1979. PMID: 376814 No abstract available.
-
Differentiation of Trypanosoma cruzi Chagas, 1909 and Trypanosoma vespertilionis Battaglia, 1904 by various lectins.Tropenmed Parasitol. 1983 Jun;34(2):89-92. Tropenmed Parasitol. 1983. PMID: 6349060
-
Isolation of the trypomastigote form of Trypanosoma cruzi from a mixture of the trypomastigote and epimastigote forms of the parasite by use of a DEAE-cellulose column.J Parasitol. 1972 Jun;58(3):631-2. J Parasitol. 1972. PMID: 4557540 No abstract available.
-
Fine structure of the trypomastigote form of Trypanosoma cruzi isolated from acellular culture by passage in column.Rev Bras Biol. 1977 Aug;37(3):671-5. Rev Bras Biol. 1977. PMID: 333521 No abstract available.
Cited by
-
Impairment of macrophage function by inhibitors of ornithine decarboxylase activity.Infect Immun. 1987 Oct;55(10):2461-4. doi: 10.1128/iai.55.10.2461-2464.1987. Infect Immun. 1987. PMID: 3115898 Free PMC article.
-
A new method for purification of Eimeria tenella merozoites.Parasitol Res. 1990;76(7):566-9. doi: 10.1007/BF00932562. Parasitol Res. 1990. PMID: 2217116
-
Arginine decarboxylase inhibitors reduce the capacity of Trypanosoma cruzi to infect and multiply in mammalian host cells.Proc Natl Acad Sci U S A. 1987 Jun;84(12):4278-82. doi: 10.1073/pnas.84.12.4278. Proc Natl Acad Sci U S A. 1987. PMID: 3295879 Free PMC article.
-
Binding of the specific ligand to Fc receptors on Trypanosoma cruzi increases the infective capacity of the parasite.Immunology. 1991 Jan;72(1):114-20. Immunology. 1991. PMID: 1900054 Free PMC article.
-
Monoclonal antibodies to Trypanosoma cruzi inhibit motility and nucleic acid synthesis of culture forms.Infect Immun. 1983 Jan;39(1):377-82. doi: 10.1128/iai.39.1.377-382.1983. Infect Immun. 1983. PMID: 6337102 Free PMC article.