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. 2023 Oct;45(10):2489-2497.
doi: 10.1002/hed.27459. Epub 2023 Jul 31.

miR-99a-5p as a biomarker for lymph node metastasis prediction in oral squamous cell carcinoma patients

Cristiane Pereira Vargas Cousseau  1 , Bruna Pereira Sorroche  1 , Renan de Jesus Teixeira  1 , Ana Carolina de Carvalho  1 , Matias Eliseo Melendez  1   2 , Renato de Castro Capuzzo  3 , Ana Carolina Laus  1 , Luciane Sussuchi da Silva  1 , Nei Soares de Menezes  4 , André Lopes Carvalho  1 , Lidia Maria Rebolho Batista Arantes  1
Affiliations

miR-99a-5p as a biomarker for lymph node metastasis prediction in oral squamous cell carcinoma patients

Cristiane Pereira Vargas Cousseau et al. Head Neck. 2023 Oct.

Abstract

Background: Metastatic lymph node involvement influences therapy decisions and serves as a prognostic indicator in oral squamous cell carcinoma (OSCC). However, many early-stage patients with clinically negative lymph nodes exhibit no metastasis upon surgical staging. This study aimed to identify differentially expressed miRNAs capable of distinguishing pathologically positive (pN+) from negative (pN0) nodes in OSCC patients without clinical evidence of lymph node metastases (cN0).

Methods: Expression levels of 798 miRNAs were assessed in tumor samples from 10 pN+ and 10 pN0 patients using the Nanostring nCounter platform. Validation was performed in an independent cohort of 15 pN+ and 24 pN0 patients through RT-qPCR.

Results: Eight miRNAs exhibited differential expression between pN0 and pN+ patients. Notably, hsa-miR-99a-5p demonstrated high sensitivity and specificity in predicting patients at higher risk of positive lymph nodes.

Conclusions: These findings highlight hsa-miR-99a-5p as a potential biomarker for detecting lymph node metastasis in primary OSCC tumors.

Keywords: biomarker; differential expression; lymphatic metastasis; miR-99a-5p; microRNAs; oral squamous cell carcinoma.

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