Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Sep;50(9):7589-7595.
doi: 10.1007/s11033-023-08703-y. Epub 2023 Aug 1.

High-fat diet-induced biogenesis of pulmonary exosomes in an experimental rat model

Maryam Shoaran  1 , Mohammad Javad Behmand  2 , Reza Rahbarghazi  3 , Reza Mosaddeghi-Heris  4 , Mahdi Ahmadi #  5 , Jafar Rezaie #  6
Affiliations

High-fat diet-induced biogenesis of pulmonary exosomes in an experimental rat model

Maryam Shoaran et al. Mol Biol Rep. 2023 Sep.

Abstract

Background: High-fat diets (HFD) have recently become a public health concern. We hypothesize that HFD induces exosomes biogenesis in the lung tissue of rat model.

Methods and results: Sixteen adult male Wistar rats were fed with HFD or a regular chow diet for 3 months. The histopathological changes in lung tissues were measured by hematoxylin and eosin (H&E) staining. Bronchoalveolar lavage (BAL) was performed to assay exosomes by acetylcholinesterase enzyme (AhCE) activity. Real-time PCR (qPCR) was used to evaluate Rab27-b, Alix, and IL-1β expression, while the immunohistochemical examination was performed for CD81 expression in lung tissues. In addition, expression of IL-1β was detected by ELISA. We found pathological alterations in the lung tissue of HFD animals. AhCE activity along with the expression level of Rab27-b, Alix, and IL-1β was increased in HFD animals (p < 0.05). Immunohistochemical staining showed that expression of CD81 was increased in lung tissues of HFD animals compared with the control group (p < 0.05).

Conclusion: Hence, HFD induced exosomes biogenesis and histopathological changes with IL-1β expression in rats' lung tissues.

Keywords: AhCE activity; Exosomes; HFD; Inflammation; Obesity.

PubMed Disclaimer

Similar articles

See all similar articles

References

    1. Canfora EE, Meex RC, Venema K, Blaak EE (2019) Gut microbial metabolites in obesity, NAFLD and T2DM. Nat Rev Endocrinol 15(5):261–273 - DOI - PubMed
    1. Buckley J (2018) Availability of high-fat foods might drive the obesity epidemic. Nat Rev Endocrinol 14(10):574–575 - DOI - PubMed
    1. Pacana T, Cazanave S, Verdianelli A, Patel V, Min H-K, Mirshahi F, Quinlivan E, Sanyal AJ (2015) Dysregulated hepatic methionine metabolism drives homocysteine elevation in diet-induced nonalcoholic fatty liver disease. PLoS ONE 10(8):e0136822 - DOI - PubMed - PMC
    1. Kim Y-C, Seok S, Byun S, Kong B, Zhang Y, Guo G, Xie W, Ma J, Kemper B, Kemper JK (2018) AhR and SHP regulate phosphatidylcholine and S-adenosylmethionine levels in the one-carbon cycle. Nat Commun 9(1):540 - DOI - PubMed - PMC
    1. Lee S, Norheim F, Gulseth HL, Langleite TM, Aker A, Gundersen TE, Holen T, Birkeland KI, Drevon CA (2018) Skeletal muscle phosphatidylcholine and phosphatidylethanolamine respond to exercise and influence insulin sensitivity in men. Sci Rep 8(1):6531 - DOI - PubMed - PMC

Substances

LinkOut - more resources