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. 2023 Sep;118(9):783-789.
doi: 10.1111/vox.13499. Epub 2023 Aug 2.

Development of an in-house bone marrow collection kit: The Catalan bone marrow transplantation group experience

Jesus Fernandez-Sojo  1   2 , Elena Valdivia  1 , Albert Esquirol  3 , Jose-Manuel Portos  3 , Montse Rovira  4 , Maria Suarez  4 , Cristina Diaz-de-Heredia  5 , Maria-Luz Uría  5 , Guillermo Ortí  6 , Christelle Ferra  7 , Alberto Mussetti  8 , Annalisa Paviglianiti  8 , Julia Marsal  9 , Isabel Badell  10 , Miquel Lozano  11 , David Gomez  1 , Carmen Azqueta  1 , Lluis Martorell  1 , Nuria Rubio  1 , Ana Garcia-Buendia  12 , Juliana Villa  13 , Enric Carreras  13 , Sergio Querol  1
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Development of an in-house bone marrow collection kit: The Catalan bone marrow transplantation group experience

Jesus Fernandez-Sojo et al. Vox Sang. 2023 Sep.

Abstract

Background and objectives: Bone marrow (BM) harvesting is one of the essential sources of stem cells for haematopoietic stem cell transplantation. In 2019, commercial BM collection kits became unavailable in Europe. Consequently, we created an in-house BM collection kit as an alternative.

Materials and methods: We compared two groups of BM collections. The first collections were taken using an in-house kit from June 2022 through February 2023 and the second with a commercial kit from February 2021 through May 2022. These all took place at seven collection centres (CC). We analysed the harvest quality (cell blood count, CD34+ cells, viability, potency and sterility), the incidents occurring with each kit and the time to neutrophil and platelet engraftment in recipients.

Results: A total of 23 donors underwent BM harvesting with the in-house kit and 23 with the commercial one. Both cohorts were comparable regarding donor characteristics, CC and time to procedure. No statistical differences were found in harvest quality between the in-house and commercial kits. A new transfusion set was required in three BM harvests (13%) with the in-house kit because of filter clogging. The median time to neutrophil and platelet engraftment was 21 days for both cohorts and 29 days (in-house) and 33 days (commercial), p = 0.284, respectively.

Conclusion: The in-house BM collection kit offers a real approach to solve the diminished supply of commercial kits. A higher risk of filter clogging was observed compared with commercial kits due to the lack of 850 and 500 μm filters.

Keywords: bone; collection; commercial; in-house; kit; marrow.

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References

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