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. 2023 Jun 30;10(2):151-156.
doi: 10.5455/javar.2023.j664. eCollection 2023 Jun.

Evaluation of antibacterial efficacy of garlic (Allium sativum) and ginger (Zingiber officinale) crude extract against multidrug-resistant (MDR) poultry pathogen

Zakaria Al Noman  1 , Tasnia Tabassum Anika  1 , Sabbya Sachi  1 , Jannatul Ferdous  1 , Yousuf Ali Sarker  1 , Md Abdus Sabur  2 , Md Tanvir Rahman  2 , Mahmudul Hasan Sikder  1
Affiliations

Evaluation of antibacterial efficacy of garlic (Allium sativum) and ginger (Zingiber officinale) crude extract against multidrug-resistant (MDR) poultry pathogen

Zakaria Al Noman et al. J Adv Vet Anim Res. .

Abstract

Objective: The study is aimed to understand the antibacterial sensitivity of native and Indian varieties of garlic (Allium sativum) and ginger (Zingiber officinale) crude extracts against multidrug-resistant (MDR) poultry pathogen (Escherichia coli and Salmonella sp.).

Materials and methods: Thin layer chromatography (TLC) is used to identify the target spices' bioactive antibacterial compounds. MDR E. coli and Salmonella sp. were isolated from poultry. The TLC-Bioautography technique was applied to explore the antibacterial potentiality of garlic and ginger.

Results: Inhibitory activities of garlic were Zone of inhibition (ZI) = 14.03 ± 0.15 mm and 19.70 ± 0.36 mm, Minimum inhibitory concentration (MIC): 0.625 and 0.325 mg/ml, and ginger were ZI = 14.63 ± 0.30 mm and 11.56 ± 0.51mm, MIC: 9.0 mg/ml against E. coli and Salmonella sp., respectively. Two bands of garlic (Rf value = 0.31 and 0.50) and one band of ginger (Rf value = 0.71) showed inhibitory potential in TLC-Bioautography against both MDR isolates.

Conclusion: Garlic and ginger were effective against MDR E. coli and Salmonella sp. These spices could be a suitable alternative during the antibiotic void.

Keywords: Multidrug-resistant pathogen; TLC-Bioautography; alternative antibiotic.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1.
Figure 1.. Phytochemical analysis by TLC-bioautography of crude extract of ginger (left panel) and garlic (right panel). Two bands (A & B) and four bands (A, B, C, and D) were separated. Only B band of ginger and B & C bands of garlic shows antibacterial activity.
See this image and copyright information in PMC

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