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. 1986 May 1;235(3):891-3.
doi: 10.1042/bj2350891.

Pig heart fumarase really does exhibit negative kinetic co-operativity at a constant ionic strength

B B Hasinoff, J P Davey

Pig heart fumarase really does exhibit negative kinetic co-operativity at a constant ionic strength

B B Hasinoff et al. Biochem J. .

Abstract

The kinetics of the action of fumarase on L-malate and fumarate were investigated at constant ionic strength. This was done to evaluate reports that fumarase follows simple Michaelis-Menten kinetics. However, when pH, buffer concentration and ionic strength are all maintained at constant values, the Lineweaver-Burk plots exhibit pronounced downward curvature, characteristic of negative kinetic co-operativity.

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