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. 2023 Sep:71:103172.
doi: 10.1016/j.scr.2023.103172. Epub 2023 Jul 28.

Generation of human induced pluripotent stem cell line EURACi015-A from a patient affected by dilated cardiomyopathy carrying the Lamin A/C p.Glu161Lys mutation

Giada Cattelan  1 , Laura Sophie Frommelt  2 , Chiara Volani  3 , Andrea Colliva  4 , Giulio Ciucci  4 , Alessia Paldino  5 , Matteo Dal Ferro  5 , Marina Di Segni  6 , Rosamaria Silipigni  6 , Peter P Pramstaller  7 , Marzia De Bortoli  7 , Serena Zacchigna  4 , Alessandra Rossini  7
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Free article

Generation of human induced pluripotent stem cell line EURACi015-A from a patient affected by dilated cardiomyopathy carrying the Lamin A/C p.Glu161Lys mutation

Giada Cattelan et al. Stem Cell Res. 2023 Sep.
Free article

Abstract

Dilated cardiomyopathy (DCM) is a common heart disorder caused by genetic and non-genetic etiologies, characterized by left ventricular dilatation and contractile dysfunction. Here, we created a human induced pluripotent stem cell line from peripheral blood mononuclear cells using non-integrating vectors from a patient carrying a heterozygous LMNA variant (c.481G > A, p.Glu161Lys, NM_170707.4). The obtained EURACi015-A line, showed the typical morphology of pluripotent cells, normal karyotype and exhibited pluripotency markers and a trilineage differentiation potential. This cell line can be successfully differentiated into cardiomyocytes and endothelial cells. This line represents a human in vitro model to study the genetic basis of DCM.

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Conflict of interest statement

Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Alessandra Rossini and Serena Zacchigna reports financial support was provided by European Regional Development Fund.

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