Isolation and characterization of high-buoyant-density proteoglycans from semilunar menisci

Abstract

Proteoglycans were extracted from adult canine menisci in high yield and were purified, and the major species were characterized biochemically. Most proteoglycans in menisci were isolated in the high-buoyant-density fraction. By agarose-acrylamide composite gel electrophoresis, two proteoglycans were seen in this fraction. Although they were smaller than those from porcine laryngeal hyaline cartilage and had shorter chondroitin sulphate and keratan sulphate chains and a lower carbohydrate-to-protein ratio, they were functionally similar to those in hyaline cartilage in their specific interaction with hyaluronate.