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. 2025 Sep;108(5):342-350.
doi: 10.1002/cyto.b.22136. Epub 2023 Aug 4.

Use of a hybrid intelligence decision tree to identify mature B-cell neoplasms

Inès Vergnolle  1 , Theo Ceccomarini  1 , Alban Canali  1 , Jean-Baptiste Rieu  1 , François Vergez  1   2   3
Affiliations

Use of a hybrid intelligence decision tree to identify mature B-cell neoplasms

Inès Vergnolle et al. Cytometry B Clin Cytom. 2025 Sep.

Abstract

Background: Mature B-cell neoplasms are challenging to diagnose due to their heterogeneity and overlapping clinical and biological features. In this study, we present a new workflow strategy that leverages a large amount of flow cytometry data and an artificial intelligence approach to classify these neoplasms.

Methods: By combining mathematical tools, such as classification algorithms and regression tree (CART) models, with biological expertise, we have developed a decision tree that accurately identifies mature B-cell neoplasms. This includes chronic lymphocytic leukemia (CLL), for which cytometry has been extensively used, as well as other non-CLL subtypes.

Results: The decision tree is easy to use and proposes a diagnosis and classification of mature B-cell neoplasms to the users. It can identify the majority of CLL cases using just three markers: CD5, CD43, and CD200.

Conclusion: This approach has the potential to improve the accuracy and efficiency of mature B-cell neoplasm diagnosis.

Keywords: artificial intelligence; classification; decision tree; mature B‐cell neoplasms.

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