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Review
. 2023 Sep:90:102030.
doi: 10.1016/j.arr.2023.102030. Epub 2023 Aug 5.

Single-cell RNA sequencing in dissecting microenvironment of age-related macular degeneration: Challenges and perspectives

Yao Tan  1 , Jianguo Huang  1 , Deshuang Li  1 , Chang Zou  2 , Dongcheng Liu  3 , Bo Qin  4
Affiliations
Review

Single-cell RNA sequencing in dissecting microenvironment of age-related macular degeneration: Challenges and perspectives

Yao Tan et al. Ageing Res Rev. 2023 Sep.

Abstract

Age-related macular degeneration (AMD) is the leading cause of blindness in individuals over the age of 50 years, yet its etiology and pathogenesis largely remain uncovered. Single-cell RNA sequencing (scRNA-seq) technologies are recently developed and have a number of advantages over conventional bulk RNA sequencing techniques in uncovering the heterogeneity of complex microenvironments containing numerous cell types and cell communications during various biological processes. In this review, we summarize the latest discovered cellular components and regulatory mechanisms during AMD development revealed by scRNA-seq. In addition, we discuss the main challenges and future directions in exploring the pathophysiology of AMD equipped with single-cell technologies. Our review underscores the importance of multimodal single-cell platforms (such as single-cell spatiotemporal multi-omics and single-cell exosome omics) as new approaches for basic and clinical AMD research in identifying biomarker, characterizing cellular responses to drug treatment and environmental stimulation.

Keywords: Age-related macular degeneration; Microenvironment; Retina; Single-cell RNA sequencing; Transcriptome.

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Conflict of interest statement

Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Bo Qin reports financial support was provided by Natural Science Foundation of Guangdong Province, China. Bo Qin reports financial support was provided by Hunan Provincial Natural Science Foundation of China. Bo Qin reports financial support was provided by Shenzhen Science and Technology Innovation Committee, China. Bo Qin reports financial support was provided by Scientific research program of Xiangjiang Philanthropy Foundation. Bo Qin reports financial support was provided by Science Research Grant of Aier Eye Hospital Group. Dongcheng Liu reports financial support was provided by Shenzhen Science and Technology Innovation Committee, China. Dongcheng Liu reports financial support was provided by Science Research Grant of Aier Eye Hospital Group.

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