Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Oct;415(24):6107-6115.
doi: 10.1007/s00216-023-04884-4. Epub 2023 Aug 8.

Determining the selectivity of a tetra-phosphorylated biomimetic peptide towards uranium in the presence of competing cations through the simultaneous coupling of HILIC to ESI-MS and ICP-MS

Lana Abou-Zeid  1   2   3 , Albert Pell  1 , Marta Garcia-Cortes  1   4 , Hélène Isnard  1 , Pascale Delangle  5 , Carole Bresson  6
Affiliations

Determining the selectivity of a tetra-phosphorylated biomimetic peptide towards uranium in the presence of competing cations through the simultaneous coupling of HILIC to ESI-MS and ICP-MS

Lana Abou-Zeid et al. Anal Bioanal Chem. 2023 Oct.

Abstract

A cyclic tetra-phosphorylated biomimetic peptide (pS1368) has been proposed as a promising starting structure to design a decorporating agent of uranyl (UO22+) due to its affinity being similar to that of osteopontin (OPN), a target UO22+ protein in vivo. The determination of this peptide's selectivity towards UO22+ in the presence of competing endogenous elements is also crucial to validate this hypothesis. In this context, the selectivity of pS1368 towards UO22+ in the presence of Ca2+, Cu2+ and Zn2+ was determined by applying the simultaneous coupling of hydrophilic interaction chromatography (HILIC) to electrospray ionization (ESI-MS) and inductively coupled plasma (ICP-MS) mass spectrometry. Sr2+ was used as Ca2+ simulant, providing less challenging ICP-MS measurements. The separation of the complexes by HILIC was first set up. The selectivity of pS1368 towards UO22+ was determined in the presence of Sr2+, by adding several proportions of the latter to UO2(pS1368). UO22+ was not displaced from UO2(pS1368) even in the presence of a ten-fold excess of Sr2+. The same approach has been undertaken to demonstrate the selectivity of pS1368 towards UO22+ in the presence of Cu2+, Zn2+ and Sr2+ as competing endogenous cations. Hence, we showed that pS1368 was selective towards UO22+ in the presence of Sr2+, but also in the presence of Cu2+ and Zn2+. This study highlights the performance of HILIC-ESI-MS/ICP-MS simultaneous coupling to assess the potential of molecules as decorporating agents of UO22+.

Keywords: Competing ions; HILIC; Mass spectrometry/ICP-MS; Phosphorylated peptide; Selectivity; Uranium.

PubMed Disclaimer

References

    1. Zoroddu MA, Aaseth J, Crisponi G, Medici S, Peana M, Nurchi VM. The essential metals for humans: a brief overview. J Inorg Biochem. 2019;195:120–9. - PubMed
    1. Ma M, Wang R, Xu L, Xu M, Liu S. Emerging health risks and underlying toxicological mechanisms of uranium contamination: lessons from the past two decades. Environ Int. 2020;145:106107. - PubMed
    1. Ansoborlo E, Prat O, Moisy P, Den Auwer C, Guilbaud P, Carriere M, et al. Actinide speciation in relation to biological processes. Biochimie. 2006;88(11):1605–18. - PubMed
    1. Garai A, Delangle P. Recent advances in uranyl binding in proteins thanks to biomimetic peptides. J Inorg Biochem. 2020;203:110936. - PubMed
    1. Ansoborlo É, Amekraz B, Moulin C, Moulin V, Taran F, Bailly T, et al. Review of actinide decorporation with chelating agents. C R Chim. 2007;10(10–11):1010–9.

LinkOut - more resources