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. 2023 Apr;52(4):840-847.
doi: 10.18502/ijph.v52i4.12457.

Polymorphism of LRP4 Gene (rs9667108) among Post Menopause Women with Osteoporosis

Saeid Kavoosian  1   2 , Alimohammad Asgharian  3 , Mohsen Asouri  2 , Sadegh Fattahi  2 , Galia Amirbozorgi  2 , Ali Kheirandish  4 , Sajad Abolfazli  4 , Maryam Lotfi  2 , Nima Ebrahimi  5 , Ramin Ataee  6   7
Affiliations

Polymorphism of LRP4 Gene (rs9667108) among Post Menopause Women with Osteoporosis

Saeid Kavoosian et al. Iran J Public Health. 2023 Apr.

Abstract

Background: Many studies have been done to identify the factors that influence the development and progression of osteoporosis. One genetic factor is polymorphisms of LRP4 gene. Regarding the lack of comprehensive study on polymorphisms of LRP4 gene in the north of Iran, mainly Mazandaran Province, we decided to investigate the polymorphism of this gene in postmenopausal women with osteoporosis.

Methods: This case-control study has been conducted at GhaemShahr Valiasr Hospital on 100 female patients with osteoporosis (average age of 58.1) and 90 healthy females without osteoporosis (average age of 55.2). After sampling and extraction of genomic DNA via of the salt deposition method, the genotype and SNP (rs9667108) polymorphism of LRP4 gene were evaluated with the PCR-RFLP method. Restriction enzymes cut the PCR products. In order to identify patients, their bone mineral density was tested by the DEXA method. The results of digestion (digestion enzyme) were analyzed by MedCalc, SPSS software, Hardy-Weinberg equilibrium, and Chi2.

Results: The statistical analysis has shown the significant relationship between SNP (rs9667108) polymorphism and the risk of osteoporosis disease in patients and control groups (P<0.05). In SNP (rs9667108), the GC genotype, compared to GG, increased the risk of disease significantly (1.556 time). Similarly, CC genotype, compared to GG genotype, increased the risk of this disease by 2.091 time.

Conclusion: The existence of mutation in the LRP4 gene could increase susceptibility to osteoporosis disease. Moreover, determining this patient's genotype in SNP (rs9667108) can be used to identify individuals who are in endanger osteoporosis.

Keywords: Osteoporosis; PCR; Polymorphism genetics; Single nucleotide polymorphisms (SNPs).

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Conflict of interest statement

Conflict of Interest The authors declare that there is no conflict of interests.

Figures

Fig. 1:
Fig. 1:
Electrophoresis of PCR product before (A) and after enzymatic digestion (B) on 2% gel. In order from left to right: −1 Ladder 500bp - 2-Undigested product - 3-Wild homozygous genotype GG (+ / +) - 4-Heterozygous genotype GC (+/−) – 5-Homozygous mutant CC (− / −) - 6 -Ladder500bp
See this image and copyright information in PMC

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