Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Dec;43(12):1916-1925.
doi: 10.1002/jat.4527. Epub 2023 Aug 8.

Characterisation of ChE in Solea solea and exposure of isoflavones in juveniles of commercial flatfish (Solea solea and Solea senegalensis)

María Gemma Albendín  1 , Vanessa Aranda  1 , Alejandro Corrales  1 , Juan Bosco Ortiz-Delgado  2 , Carmen Sarasquete  2 , Juana María Arellano  1
Affiliations

Characterisation of ChE in Solea solea and exposure of isoflavones in juveniles of commercial flatfish (Solea solea and Solea senegalensis)

María Gemma Albendín et al. J Appl Toxicol. 2023 Dec.

Abstract

The isoflavones genistein and daidzein are flavonoid compounds mainly found in legumes, especially in soybeans and their derived products. These flavonoids can be present in agricultural, domestic and industrial wastewater effluents as a result of anthropogenic activities and may be discharged in the environment. Due to the large growth of the aquaculture sector in recent decades, new and cost-effective fish feeds are being sought, but there is also a particular need to determine the effects of exposed flavonoids on fish in the aquatic environment, as this is the main route of exposure of organisms to endocrine disruptors. This study evaluated the possible effects of these isoflavones on juveniles of Solea senegalensis and Solea solea. After 48-96 h of exposure, the acetylcholinesterase activity in the sole head tissues was measured, and the cholinesterase activity in juveniles of common sole (S. solea) was determined. Experiments were carried out to determine the optimal pH, investigate the specificity of three substrates (acetylthiocholine, butyrylthiocholine, propionylthiocholine) on cholinesterase activity and determine the kinetic parameters (Vmax and Km ). The results obtained showed that neither genistein nor daidzein exposure to S. senegalensis and S. solea inhibited the activity of acetylcholinesterase at the tested concentrations (genistein: 1.25, 2.5, 5, 10 and 20 mg/L; daidzein: 0.625, 1.25, 2.5, 5 and 10 mg/L).

Keywords: acetylcholinesterase; daidzein; genistein; isoflavones; sole.

PubMed Disclaimer

References

REFERENCES

    1. Ahmad, A., Kurniawan, S. B., Abdullah, S. R. S., Othman, A. R., & Hasan, H. A. (2022). Contaminants of emerging concern (CECs) in aquaculture effluent: Insight into breeding and rearing activities, alarming impacts, regulations, performance of wastewater treatment unit and future approaches. Chemosphere, 229, 133319. https://doi.org/10.1016/j
    1. Albendín, G., Arellano, J. M., Mánuel-Vez, M. P., Sarasquete, C., & Arufe, M. I. (2017). Characterization and in vitro sensitivity of cholinesterases of gilthead seabream (Sparus aurata) to organophosphate pesticides. Fish Physiology and Biochemistry, 43, 455-464. https://doi.org/10.1007/s10695-016-0299-y
    1. Arufe, M. I., Arellano, J. M., García, L., Albendín, G., & Sarasquete, C. (2007). Cholinesterase activity in gilthead seabream (Sparus aurata) larvae: Characterization and sensitivity to the organophosphate azinphosmethyl. Aquatic Toxicology, 84(3), 328-336. https://doi.org/10.1016/j.aquatox.2007.06.009
    1. Bennetau-Pelissero, C., Breton B, B., Bennetau, B., Corraze, G., Le Menn, F., Davail-Cuisset, B., Helou, C., & Kaushik, S. J. (2001). Effect of genistein-enriched diets on the endocrine process of gametogenesis and on reproduction efficiency of the rainbow trout Oncorhynchus mykiss. General Comparative Endocrinology, 121(2), 173-187. https://doi.org/10.1006/gcen.2000.7585
    1. Botté, E. S., Jerry, D. R., King, S. C., Smith-keune, C., & Negri, A. P. (2012). Effects of chlorpyrifos on cholinesterase activity and stress markers in the tropical reef fish Acanthochromis polyacanthus. Marine Pollution Bulletin, 65, 384-393. https://doi.org/10.1016/j.marpolbul.2011.08.020

Publication types

LinkOut - more resources